Concentrations of HX and TdR in the Culture Medium of Leukemic Blast Samples
| Sample No. . | In the Absence of ALL Cells . | In the Presence of ALL Cells . | ||||||
|---|---|---|---|---|---|---|---|---|
| Hx (μmol/L) . | TdR (μmol/L) . | Hx (μmol/L) . | TdR (μmol/L) . | |||||
| −XO . | +XO . | −TP . | +TP . | −XO . | +XO . | −TP . | +TP . | |
| 1 | 67.8 | <0.4 | 1.43 | <0.1 | 58.7 | 0.4 | 2.09 | <0.1 |
| 2 | 56.6 | <0.4 | 0.43 | <0.1 | 46.5 | <0.4 | 0.82 | <0.1 |
| 3 | ND | ND | ND | ND | 41.5 | 14.6 | <0.1 | <0.1 |
| 4 | 37.2 | <0.4 | <0.1 | <0.1 | 36.4 | <0.4 | 0.22 | <0.1 |
| Sample No. . | In the Absence of ALL Cells . | In the Presence of ALL Cells . | ||||||
|---|---|---|---|---|---|---|---|---|
| Hx (μmol/L) . | TdR (μmol/L) . | Hx (μmol/L) . | TdR (μmol/L) . | |||||
| −XO . | +XO . | −TP . | +TP . | −XO . | +XO . | −TP . | +TP . | |
| 1 | 67.8 | <0.4 | 1.43 | <0.1 | 58.7 | 0.4 | 2.09 | <0.1 |
| 2 | 56.6 | <0.4 | 0.43 | <0.1 | 46.5 | <0.4 | 0.82 | <0.1 |
| 3 | ND | ND | ND | ND | 41.5 | 14.6 | <0.1 | <0.1 |
| 4 | 37.2 | <0.4 | <0.1 | <0.1 | 36.4 | <0.4 | 0.22 | <0.1 |
ALL samples (2 × 106 cells/mL) were incubated in 96-well plates for 4 days in the absence or presence of XO (final concentration, 0.02 IU/mL) and TP (final concentration, 0.15 IU/mL). The concentrations of TdR and HX were measured by HPLC as described in Materials and Methods.
Abbreviation: ND, not determined.