Effect of Leukotriene Receptor Antagonists on NO Formation in PMN
Percent Reduction of NO Production Stimulus (at 100 nmol/L) . | |||
---|---|---|---|
Inhibitor | LTD4 | LTC4 | LTB4 |
SK&F 104 353 | 86.6 ± 18.5 | 70.8 ± 19.9 | 0 |
CP-105,696 | 0 | 0 | 79.0 ± 12.2 |
Percent Reduction of NO Production Stimulus (at 100 nmol/L) . | |||
---|---|---|---|
Inhibitor | LTD4 | LTC4 | LTB4 |
SK&F 104 353 | 86.6 ± 18.5 | 70.8 ± 19.9 | 0 |
CP-105,696 | 0 | 0 | 79.0 ± 12.2 |
Inhibition of the PMN-derived NO release activated by LTB4, LTD4, or LTC4 after treatment with SK&F 104 353 or CP-105,696 (both at 10 μmol/L), as percent of similarly treated PMN, but without the specific receptor antagonist. The release of NO from activated PMN, without receptor inhibitors, was for LTB4 232 ± 50, for LTC4240 ± 99, and for LTD4 145 ± 67 pmol/106PMN. The NO release was measured at 30 minutes after activation and performed as detailed in Materials and Methods section. Mean ± SEM; n = 4.