Flow Cytometry Analysis of the Effect IL-2 Addition on Reversing the Effect of IL-10 on SEB-Stimulated CD4+ T Cells: Two Day IL-10 Pretreatment
| IL-2 (U/mL) . | IL-10 . | SEB . | Cell Cycle Distribution (%)7-150 . | ||
|---|---|---|---|---|---|
| G1 . | S . | G2/M . | |||
| 0 | − | − | >98 | <1 | <1 |
| 0 | − | + | 73.70 | 23.20 | 3.10 |
| 100 | + | + | 61.20 | 33.20 | 5.60 |
| 30 | + | + | 62.10 | 32.60 | 5.30 |
| 10 | + | + | 79.50 | 16.00 | 4.50 |
| 3 | + | + | 88.30 | 9.50 | 2.20 |
| 0 | + | + | 89.20 | 8.70 | 2.00 |
| 100 | − | + | 53.40 | 41.20 | 5.40 |
| 3 | − | + | 70.80 | 24.70 | 4.50 |
| 100 | + | − | 80.80 | 16.80 | 2.40 |
| 3 | + | − | 93.30 | 5.50 | 1.10 |
| 100 | − | − | 65.70 | 29.40 | 4.90 |
| 3 | − | − | 74.00 | 23.80 | 2.20 |
| IL-2 (U/mL) . | IL-10 . | SEB . | Cell Cycle Distribution (%)7-150 . | ||
|---|---|---|---|---|---|
| G1 . | S . | G2/M . | |||
| 0 | − | − | >98 | <1 | <1 |
| 0 | − | + | 73.70 | 23.20 | 3.10 |
| 100 | + | + | 61.20 | 33.20 | 5.60 |
| 30 | + | + | 62.10 | 32.60 | 5.30 |
| 10 | + | + | 79.50 | 16.00 | 4.50 |
| 3 | + | + | 88.30 | 9.50 | 2.20 |
| 0 | + | + | 89.20 | 8.70 | 2.00 |
| 100 | − | + | 53.40 | 41.20 | 5.40 |
| 3 | − | + | 70.80 | 24.70 | 4.50 |
| 100 | + | − | 80.80 | 16.80 | 2.40 |
| 3 | + | − | 93.30 | 5.50 | 1.10 |
| 100 | − | − | 65.70 | 29.40 | 4.90 |
| 3 | − | − | 74.00 | 23.80 | 2.20 |
PBMCs were treated for 2 days with IL-10 (10 U/mL). They were then incubated with SEB (100 pg/mL) and IL-2 at the indicated concentrations for 5.5 days before flow cytometry analysis. Data were gated to analyze CD4+ T cells.