Reactivity of Human Alloantibodies and Autoantibodies in the MAIGA Assay with Transfected CHO Cells and Human Polymorphonuclear (PMN) Leukocytes Expressing Different Isoforms of the FcγRIIIb
| Alloantibodies . | CHO-NA1 . | CHO-NA2 . | CHO-SH . | CHO-WT . | PMN-NA1 . | PMN-NA2 . | PMN-SH . |
|---|---|---|---|---|---|---|---|
| Anti-NA1 | 4/5 | 0/5 | 0/5 | 0/5 | 4/5 | 0/5 | 0/5 |
| Anti-NA2 | 0/5 | 5/5 | 5/5 | 0/5 | 0/5 | 5/5 | 5/5 |
| Anti-SH | 0/4 | 0/4 | 3/4 | 0/4 | 0/4 | 0/4 | 3/4 |
| Anti-FcγRIIIb | 5/5 | 5/5 | 5/5 | 0/5 | 5/5 | 5/5 | 5/5 |
| Anti-SAR | + | + | + | − | + | + | + |
| Anti-LAN | + | + | + | − | + | + | + |
| Autoantibodies | 12/12 | 6/12 | 11/12 | 0/12 | 12/12 | 6/12 | 11/12 |
| Alloantibodies . | CHO-NA1 . | CHO-NA2 . | CHO-SH . | CHO-WT . | PMN-NA1 . | PMN-NA2 . | PMN-SH . |
|---|---|---|---|---|---|---|---|
| Anti-NA1 | 4/5 | 0/5 | 0/5 | 0/5 | 4/5 | 0/5 | 0/5 |
| Anti-NA2 | 0/5 | 5/5 | 5/5 | 0/5 | 0/5 | 5/5 | 5/5 |
| Anti-SH | 0/4 | 0/4 | 3/4 | 0/4 | 0/4 | 0/4 | 3/4 |
| Anti-FcγRIIIb | 5/5 | 5/5 | 5/5 | 0/5 | 5/5 | 5/5 | 5/5 |
| Anti-SAR | + | + | + | − | + | + | + |
| Anti-LAN | + | + | + | − | + | + | + |
| Autoantibodies | 12/12 | 6/12 | 11/12 | 0/12 | 12/12 | 6/12 | 11/12 |