Effect of SB203580 on O2−Release in Human Neutrophils Stimulated by GM-CSF or TNF
| Pretreatment . | O2− Release (% control) . | |
|---|---|---|
| GM-CSF . | TNF . | |
| SB203580 | ||
| 0.01 μmol/L | 62.8 ± 7.3* | 66.2 ± 1.3* |
| 0.1 μmol/L | 35.2 ± 7.9* | 39.6 ± 3.4* |
| 1 μmol/L | 31.5 ± 9.5* | 32.2 ± 5.6* |
| 10 μmol/L | 32.6 ± 8.8* | 19.8 ± 3.8* |
| Pretreatment . | O2− Release (% control) . | |
|---|---|---|
| GM-CSF . | TNF . | |
| SB203580 | ||
| 0.01 μmol/L | 62.8 ± 7.3* | 66.2 ± 1.3* |
| 0.1 μmol/L | 35.2 ± 7.9* | 39.6 ± 3.4* |
| 1 μmol/L | 31.5 ± 9.5* | 32.2 ± 5.6* |
| 10 μmol/L | 32.6 ± 8.8* | 19.8 ± 3.8* |
Cells (2 × 105/0.2 mL) were preincubated with indicated concentrations of SB203580 for 20 minutes at 37°C and thereafter stimulated with GM-CSF (5 ng/mL) or TNF (100 units/mL) for 3 hours at 37°C. O2− release was determined as superoxide dismutase-inhibitable reduction of ferricytochromec during stimulation with GM-CSF or TNF. In this experiment, the amounts of O2− release in control cells stimulated by GM-CSF and TNF were 2.53 ± 0.16 and 4.92 ± 0.35 nmol/3 hours/ 2 × 105 cells, respectively. The data are expressed as the mean ± SD of three experiments.
Significantly inhibited by SB203580 (P < .001).