Expression of D3 RNA in Hematopoietic Cell Lines
Cell Line . | Lineage . | D3 . |
---|---|---|
EML | Multipotential progenitor, SCF dependent | − |
MPRO | Promyelocyte, GM-CSF dependent | ++ |
EPRO | Promyelocyte, GM-CSF dependent | ++ |
FDC-P1 (IL-3) | Myeloid progenitor, IL-3 dependent | + |
32D.Cl3 | Myeloid progenitor, IL-3 dependent | − |
32D.Cl23 | Myeloid progenitor, IL-3 dependent | − |
DA-3 | Myeloid progenitor, IL-3 dependent | − |
NFS-58 | Myeloid progenitor, IL-3 dependent | ++ |
FDC-P1 (IL-4) | Myeloid progenitor, IL-4 dependent | − |
M-NFS-60 | Myeloid progenitor, M-CSF dependent | − |
EL-4 | T lymphoma, factor independent | − |
CTLL-2 | T cytotoxic, IL-2 dependent | − |
HCD-57 | Erythroleukemia, EPO dependent | − |
M1 | Myelomonocytic leukemia | − |
Wehi-3b | Myelomonocytic leukemia | +++ |
GG2EE | Macrophage | − |
GG2EE + IFNγ | + | |
NIH 3T3 | Fibroblast | − |
Cell Line . | Lineage . | D3 . |
---|---|---|
EML | Multipotential progenitor, SCF dependent | − |
MPRO | Promyelocyte, GM-CSF dependent | ++ |
EPRO | Promyelocyte, GM-CSF dependent | ++ |
FDC-P1 (IL-3) | Myeloid progenitor, IL-3 dependent | + |
32D.Cl3 | Myeloid progenitor, IL-3 dependent | − |
32D.Cl23 | Myeloid progenitor, IL-3 dependent | − |
DA-3 | Myeloid progenitor, IL-3 dependent | − |
NFS-58 | Myeloid progenitor, IL-3 dependent | ++ |
FDC-P1 (IL-4) | Myeloid progenitor, IL-4 dependent | − |
M-NFS-60 | Myeloid progenitor, M-CSF dependent | − |
EL-4 | T lymphoma, factor independent | − |
CTLL-2 | T cytotoxic, IL-2 dependent | − |
HCD-57 | Erythroleukemia, EPO dependent | − |
M1 | Myelomonocytic leukemia | − |
Wehi-3b | Myelomonocytic leukemia | +++ |
GG2EE | Macrophage | − |
GG2EE + IFNγ | + | |
NIH 3T3 | Fibroblast | − |
RNA was extracted from the indicated cell lines for Northern blots that were probed with D3 according to the procedures outlined in Materials and Methods. Blots were reprobed with G3PDH to verify equal loading in all samples. No expression (−), low expression (+), and moderate/high expression (++/+++) of D3 are indicated.