Table 2. PCR Primers and Fragments Used... | American Society of Hematology

Table 2.

PCR Primers and Fragments Used in XCIP Analysis of the IDS, p55, and G6PD Loci

Gene	Primers^*	Location	Fragment (bp)	Enzyme	Digest (bp)
IDS
UD	5′-GCCCCAAAGAAGGGAGGGTCC-3′	Intron 3	160	Bcl I	160 + 130
DD	5′-TGGAAAAGACCAGCTATACGGAGAATGATC-3′	Exon 4
IDS
UR	5′-CTACTGGAGGGTGCACGCTGG-3′	Exon 3	148	Bcl I	148 + 118
DR	5′-TGGAAAAGACCAGCTATACGGAGAATGATC-3′	Exon 4
p55
UD	5′-CTCCTCAAAGCAGGCTTCATGCCTG-3′	Intron 1	191	Bst UI	191 + 161
DD	CGTACAGGACTGTTTTTCATTCAGCTTCCG-3′	Exon 3
p55
UR	5′-CACAGAAGAGCCCATGGGAATCGC-3′	Exon 3	178	Hin P1 I	178 + 155
DR	5′-CGCCTTCTGCAGCTGATCCAC-3′	Exon 4
G6PD
U	5′-CAACCCCGAGGAGTCGGAGCTG-3′	Exon 10	200 (DNA)	Mlu I	200 + 174
D	5′-AGAAGACGTCCAGGATGAGGCACGC-3′	Exon 11	98 (RNA)		98 + 72

Gene	Primers^*	Location	Fragment (bp)	Enzyme	Digest (bp)
IDS
UD	5′-GCCCCAAAGAAGGGAGGGTCC-3′	Intron 3	160	Bcl I	160 + 130
DD	5′-TGGAAAAGACCAGCTATACGGAGAATGATC-3′	Exon 4
IDS
UR	5′-CTACTGGAGGGTGCACGCTGG-3′	Exon 3	148	Bcl I	148 + 118
DR	5′-TGGAAAAGACCAGCTATACGGAGAATGATC-3′	Exon 4
p55
UD	5′-CTCCTCAAAGCAGGCTTCATGCCTG-3′	Intron 1	191	Bst UI	191 + 161
DD	CGTACAGGACTGTTTTTCATTCAGCTTCCG-3′	Exon 3
p55
UR	5′-CACAGAAGAGCCCATGGGAATCGC-3′	Exon 3	178	Hin P1 I	178 + 155
DR	5′-CGCCTTCTGCAGCTGATCCAC-3′	Exon 4
G6PD
U	5′-CAACCCCGAGGAGTCGGAGCTG-3′	Exon 10	200 (DNA)	Mlu I	200 + 174
D	5′-AGAAGACGTCCAGGATGAGGCACGC-3′	Exon 11	98 (RNA)		98 + 72

UD and UR denote the upstream primer for DNA and RNA analysis, respectively, and DD and DR denote the corresponding downstream primers. For G6PD, the same primers were used for both analyses. Mismatched nucleotides to introduce enzyme digestion sites in one allele are underlined.

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