Binding Properties of G-CSFR Transfectants
| G-CSFR . | Cell . | Kd (pM) . | Receptors/cell . |
|---|---|---|---|
| WT | Ba/F3 | 159 to 340 | 2.5 to 4.1 × 103 |
| COS-7 | 89 to 119 | 4.2 to 12.1 × 105 | |
| Δ716 | Ba/F3 | 75 to 606 | 4.6 to 16.4 × 103 |
| COS-7 | 42 to 91 | 2.2 to 15.9 × 105 |
| G-CSFR . | Cell . | Kd (pM) . | Receptors/cell . |
|---|---|---|---|
| WT | Ba/F3 | 159 to 340 | 2.5 to 4.1 × 103 |
| COS-7 | 89 to 119 | 4.2 to 12.1 × 105 | |
| Δ716 | Ba/F3 | 75 to 606 | 4.6 to 16.4 × 103 |
| COS-7 | 42 to 91 | 2.2 to 15.9 × 105 |
Scatchard analyses were performed on Ba/F3 and COS-7 cells transfected with either WT G-CSFR or Δ716 G-CSFR forms. Binding affinities and receptor numbers/cell are indicated. The data were generated from five independent experiments.