Activation of the HIV-1 LTR by Neutrophils and Opsonized Zymosan
| Additions . | Relative Light Units . |
|---|---|
| Background | 16,158 ± 2,641 (13)* |
| Neutrophils | 18,407 ± 3,115 (13) |
| OZ | 16,809 ± 2,831 (13) |
| Neutrophils + OZ | 26,578 ± 5,728 (13) |
| Neutrophils + OZ + catalase | 22,204 ± 4,671 (10) |
| Neutrophils + OZ + heated catalase | 22,262 ± 19,495 (10) |
| Neutrophils + OZ + azide | 252,483 ± 104,399 (7) |
| Neutrophils + OZ + azide + catalase | 39,142 ± 6,483 (7) |
| Neutrophils + OZ + azide + heated catalase | 261,413 ± 96,761 (7) |
| Additions . | Relative Light Units . |
|---|---|
| Background | 16,158 ± 2,641 (13)* |
| Neutrophils | 18,407 ± 3,115 (13) |
| OZ | 16,809 ± 2,831 (13) |
| Neutrophils + OZ | 26,578 ± 5,728 (13) |
| Neutrophils + OZ + catalase | 22,204 ± 4,671 (10) |
| Neutrophils + OZ + heated catalase | 22,262 ± 19,495 (10) |
| Neutrophils + OZ + azide | 252,483 ± 104,399 (7) |
| Neutrophils + OZ + azide + catalase | 39,142 ± 6,483 (7) |
| Neutrophils + OZ + azide + heated catalase | 261,413 ± 96,761 (7) |
The reaction mixture contained 2 × 106 Jurkat LTRluc, and where indicated, 106neutrophils/mL, 1 mg/mL opsonized zymosan (OZ), 3 × 10−5 mol/L azide, 8.2 μg/mL catalase, and 8.2 μg/mL heated catalase in RPMI at a final volume of 2.0 mL.
Mean ± SE of (n) experiments.