Table 4. Single-Cell Colony Formation of... | American Society of Hematology

Table 4.

Single-Cell Colony Formation of Lin⁻c-Kit⁺Sca-1^+/−Cells

Mice	Phenotype	Cytokines	No. of Sorted Cells	No. of Colonies
Mice	Phenotype	Cytokines	No. of Sorted Cells	GM	Mk	Mast	Blast	Mix	Total
Littermates	Lin⁻c-Kit⁺Sca-1⁺	hG-CSF	192	8	0	0	0	0	8
		mIL-3	192	68	2	7	23	13	113
	Lin⁻c-Kit⁺Sca-1⁻	hG-CSF	192	39	0	0	0	0	39
		mIL-3	192	83	7	6	0	6	102
Tg mice	Lin⁻c-Kit⁺Sca-1⁺	hG-CSF	384	117	20	10	44	18	209
		mIL-3	384	143	6	15	49	24	237
	Lin⁻c-Kit⁺Sca-1⁻	hG-CSF	384	136	14	3	0	5	158
		mIL-3	384	151	13	12	0	13	189

Mice	Phenotype	Cytokines	No. of Sorted Cells	No. of Colonies
Mice	Phenotype	Cytokines	No. of Sorted Cells	GM	Mk	Mast	Blast	Mix	Total
Littermates	Lin⁻c-Kit⁺Sca-1⁺	hG-CSF	192	8	0	0	0	0	8
		mIL-3	192	68	2	7	23	13	113
	Lin⁻c-Kit⁺Sca-1⁻	hG-CSF	192	39	0	0	0	0	39
		mIL-3	192	83	7	6	0	6	102
Tg mice	Lin⁻c-Kit⁺Sca-1⁺	hG-CSF	384	117	20	10	44	18	209
		mIL-3	384	143	6	15	49	24	237
	Lin⁻c-Kit⁺Sca-1⁻	hG-CSF	384	136	14	3	0	5	158
		mIL-3	384	151	13	12	0	13	189

Lin⁻c-Kit⁺Sca-1^+/− cells were sorted from BM of hG-CSFR-Tg mice and littermates and cultured for 8 days in the presence of hG-CSF (20 ng/mL) or mIL-3 (10 ng/mL). Representative data from the two separate experiments are shown.

View Large

This Feature Is Available To Subscribers Only