Numbers of DC Generated From Adherent PBMNC
| Cytokines* . | %DC . | (Absolute Numbers, ×103)† . | %CD3+ . | %CD19+ . |
|---|---|---|---|---|
| Flt3L/IL-4 | 31 ± 5.8 | (249 ± 73) | 4 ± 1.2 | 1 ± 1.6 |
| Flt3L/IL-4/TNF-α | 73 ± 9.1 | (884 ± 65) | 3 ± 1.7 | 1 ± 1 |
| Flt3L/IL-4/CD40L | 75 ± 7.0 | (1202 ± 211)‡ | 6 ± 3.1 | 2 ± 1.5 |
| CD40L | 55 ± 10 | (747 ± 102) | 4 ± 1.5 | 1 ± 0.8 |
| CD40L/IL-4 | 71 ± 9.0 | (1087 ± 153)1-153 | 5 ± 1.7 | 4 ± 1 |
| CD40L/IL-4/GM-CSF | 81 ± 11 | (1345 ± 241)1-155 | 2 ± 1.6 | 1 ± 0.6 |
| GM-CSF/IL-4/TNF-α | 85 ± 9.8 | (1276 ± 96) | 6 ± 1.4 | 1 ± 1.6 |
| GM-CSF/IL-4 | 76 ± 8.2 | (964 ± 120) | 4 ± 1.8 | 2 ± 1.2 |
| Cytokines* . | %DC . | (Absolute Numbers, ×103)† . | %CD3+ . | %CD19+ . |
|---|---|---|---|---|
| Flt3L/IL-4 | 31 ± 5.8 | (249 ± 73) | 4 ± 1.2 | 1 ± 1.6 |
| Flt3L/IL-4/TNF-α | 73 ± 9.1 | (884 ± 65) | 3 ± 1.7 | 1 ± 1 |
| Flt3L/IL-4/CD40L | 75 ± 7.0 | (1202 ± 211)‡ | 6 ± 3.1 | 2 ± 1.5 |
| CD40L | 55 ± 10 | (747 ± 102) | 4 ± 1.5 | 1 ± 0.8 |
| CD40L/IL-4 | 71 ± 9.0 | (1087 ± 153)1-153 | 5 ± 1.7 | 4 ± 1 |
| CD40L/IL-4/GM-CSF | 81 ± 11 | (1345 ± 241)1-155 | 2 ± 1.6 | 1 ± 0.6 |
| GM-CSF/IL-4/TNF-α | 85 ± 9.8 | (1276 ± 96) | 6 ± 1.4 | 1 ± 1.6 |
| GM-CSF/IL-4 | 76 ± 8.2 | (964 ± 120) | 4 ± 1.8 | 2 ± 1.2 |
107 PBMNC were stimulated with the indicated combinations of cytokines or by CD40 ligation for 7 days.
The percentage of DC (cells expressing CD1a and/or CD83), CD3+, and CD19+ cells was analyzed by flow cytometry. The absolute number of DC was calculated based on FACS data. Results show the means and standard deviations of six different experiments.
P < .0001 compared with Flt3L/IL-4.
P < .0001 compared with Flt3L/IL-4.
P = .0061 compared with GM-CSF/IL-4.