Growth Kinetics of Single CD34+lin− Cells in Cultures Supplemented With FLT-3L, SCF, IL-3, IL-6, G-CSF, βNGF
| Day . | % Wells With 1 Live Cell . | % Wells in Which Cell Doubled . | % Wells in Which Cell Died . |
|---|---|---|---|
| 1 | 100 | — | — |
| 2 | 88.3 ± 2.77 | 11.7 ± 2.77 | 0 ± 0 |
| 3 | 52.4 ± 9.12 | 17.8 ± 6.04 | 18.1 ± 11.08 |
| 4 | 27.4 ± 5.96 | 7.8 ± 3.03 | 17.3 ± 4.24 |
| 5 | 18.7 ± 4.28 | 2.0 ± 1.36 | 6.7 ± 1.01 |
| 6 | 10.3 ± 2.60 | 0 ± 0 | 8.4 ± 3.72 |
| 7 | 5.2 ± 2.86 | 0 ± 0 | 5.0 ± 1.09 |
| Day . | % Wells With 1 Live Cell . | % Wells in Which Cell Doubled . | % Wells in Which Cell Died . |
|---|---|---|---|
| 1 | 100 | — | — |
| 2 | 88.3 ± 2.77 | 11.7 ± 2.77 | 0 ± 0 |
| 3 | 52.4 ± 9.12 | 17.8 ± 6.04 | 18.1 ± 11.08 |
| 4 | 27.4 ± 5.96 | 7.8 ± 3.03 | 17.3 ± 4.24 |
| 5 | 18.7 ± 4.28 | 2.0 ± 1.36 | 6.7 ± 1.01 |
| 6 | 10.3 ± 2.60 | 0 ± 0 | 8.4 ± 3.72 |
| 7 | 5.2 ± 2.86 | 0 ± 0 | 5.0 ± 1.09 |
Single CD34+lin− cells were plated at 1 cell/well in serum-free medium and monitored over 7 days as described in Materials and Methods. Data represent the means ± SEM of 3 experiments with a total of 193 wells containing a single cell.