Growth Kinetics of Single CD34+lin− Cells in Cultures Supplemented With HGF
| Day . | % Wells With 1 Live Cell . | % Wells in Which Cell Doubled . | % Wells in Which Cell Died . |
|---|---|---|---|
| 1 | 100 | — | — |
| 2 | 94.8 ± 5.25 | 2.7 ± 2.65 | 2.7 ± 2.65 |
| 3 | 78.5 ± 4.80 | 11.0 ± 5.70 | 10.8 ± 0.30 |
| 4 | 56.8 ± 1.20 | 5.3 ± 5.25 | 16.6 ± 11.25 |
| 5 | 43.0 ± 9.65 | 2.8 ± 2.80 | 11.0 ± 5.70 |
| 6 | 27.1 ± 0.75 | 0 ± 0 | 16.0 ± 10.35 |
| 7 | 16.1 ± 5.00 | 0 ± 0 | 11.0 ± 5.70 |
| Day . | % Wells With 1 Live Cell . | % Wells in Which Cell Doubled . | % Wells in Which Cell Died . |
|---|---|---|---|
| 1 | 100 | — | — |
| 2 | 94.8 ± 5.25 | 2.7 ± 2.65 | 2.7 ± 2.65 |
| 3 | 78.5 ± 4.80 | 11.0 ± 5.70 | 10.8 ± 0.30 |
| 4 | 56.8 ± 1.20 | 5.3 ± 5.25 | 16.6 ± 11.25 |
| 5 | 43.0 ± 9.65 | 2.8 ± 2.80 | 11.0 ± 5.70 |
| 6 | 27.1 ± 0.75 | 0 ± 0 | 16.0 ± 10.35 |
| 7 | 16.1 ± 5.00 | 0 ± 0 | 11.0 ± 5.70 |
Single CD34+lin− cells were plated at 1 cell/well in serum-free medium and monitored over 7 days as described in Materials and Methods. Data represent the means ± SEM of 2 experiments with a total of 37 wells containing a single cell.