Growth Kinetics of Single CD34+lin− Cells in Cultures Supplemented With IL-11
| Day . | % Wells With 1 Live Cell . | % Wells in Which Cell Doubled . | % Wells in Which Cell Died . |
|---|---|---|---|
| 1 | 100 | — | — |
| 2 | 90.9 ± 0.40 | 2.2 ± 2.15 | 6.9 ± 2.60 |
| 3 | 72.3 ± 10.35 | 11.5 ± 2.85 | 7.2 ± 7.15 |
| 4 | 49.7 ± 6.80 | 4.3 ± 4.30 | 18.2 ± 0.80 |
| 5 | 35.8 ± 12.00 | 0 ± 0 | 13.8 ± 5.20 |
| 6 | 22.7 ± 1.05 | 0 ± 0 | 13.0 ± 13.00 |
| 7 | 4.8 | 0 | 19.0 |
| Day . | % Wells With 1 Live Cell . | % Wells in Which Cell Doubled . | % Wells in Which Cell Died . |
|---|---|---|---|
| 1 | 100 | — | — |
| 2 | 90.9 ± 0.40 | 2.2 ± 2.15 | 6.9 ± 2.60 |
| 3 | 72.3 ± 10.35 | 11.5 ± 2.85 | 7.2 ± 7.15 |
| 4 | 49.7 ± 6.80 | 4.3 ± 4.30 | 18.2 ± 0.80 |
| 5 | 35.8 ± 12.00 | 0 ± 0 | 13.8 ± 5.20 |
| 6 | 22.7 ± 1.05 | 0 ± 0 | 13.0 ± 13.00 |
| 7 | 4.8 | 0 | 19.0 |
Single CD34+lin− cells were plated at 1 cell/well in serum-free medium and monitored over 7 days as described in Materials and Methods. Data represent the means ± SEM of 2 experiments with a total of 44 wells containing a single cell.