Growth Kinetics of Single CD34+lin− Cells in Cultures Supplemented With IL-6
| Day . | % Wells With 1 Live Cell . | % Wells in Which Cell Doubled . | % Wells in Which Cell Died . |
|---|---|---|---|
| 1 | 100 | — | — |
| 2 | 86.0 ± 3.0 | 6.2 ± 1.80 | 7.7 ± 2.23 |
| 3 | 61.0 ± 9.3 | 6.6 ± 3.36 | 18.5 ± 9.31 |
| 4 | 35.2 ± 10.3 | 4.0 ± 0.88 | 20.4 ± 7.40 |
| 5 | 23.7 ± 8.5 | 2.5 ± 2.46 | 9.0 ± 2.70 |
| 6 | 17.8 ± 4.3 | 0 ± 0 | 5.9 ± 4.53 |
| 7 | 8.3 | 0 | 5.6 |
| Day . | % Wells With 1 Live Cell . | % Wells in Which Cell Doubled . | % Wells in Which Cell Died . |
|---|---|---|---|
| 1 | 100 | — | — |
| 2 | 86.0 ± 3.0 | 6.2 ± 1.80 | 7.7 ± 2.23 |
| 3 | 61.0 ± 9.3 | 6.6 ± 3.36 | 18.5 ± 9.31 |
| 4 | 35.2 ± 10.3 | 4.0 ± 0.88 | 20.4 ± 7.40 |
| 5 | 23.7 ± 8.5 | 2.5 ± 2.46 | 9.0 ± 2.70 |
| 6 | 17.8 ± 4.3 | 0 ± 0 | 5.9 ± 4.53 |
| 7 | 8.3 | 0 | 5.6 |
Single CD34+lin− cells were plated at 1 cell/well in serum-free medium and monitored over 7 days as described in Materials and Methods. Data represent the means ± SEM of 3 experiments with a total of 99 wells containing a single cell.