Growth Kinetics of Single CD34+lin− Cells in Cultures Supplemented With FLT-3L
| Day . | % Wells With 1 Live Cell . | % Wells in Which Cell Doubled . | % Wells in Which Cell Died . |
|---|---|---|---|
| 1 | 100 | — | — |
| 2 | 86.4 ± 1.42 | 6.8 ± 2.29 | 7.0 ± 3.55 |
| 3 | 65.1 ± 4.80 | 11.3 ± 3.59 | 9.8 ± 4.16 |
| 4 | 49.0 ± 4.04 | 4.6 ± 2.83 | 11.6 ± 4.87 |
| 5 | 38.8 ± 5.32 | 0.8 ± 0.80 | 9.4 ± 4.13 |
| 6 | 28.6 ± 4.66 | 0 ± 0 | 10.2 ± 2.06 |
| 7 | 21.8 ± 4.783-150 | 0.8 ± 0.78 | 6.1 ± 2.11 |
| Day . | % Wells With 1 Live Cell . | % Wells in Which Cell Doubled . | % Wells in Which Cell Died . |
|---|---|---|---|
| 1 | 100 | — | — |
| 2 | 86.4 ± 1.42 | 6.8 ± 2.29 | 7.0 ± 3.55 |
| 3 | 65.1 ± 4.80 | 11.3 ± 3.59 | 9.8 ± 4.16 |
| 4 | 49.0 ± 4.04 | 4.6 ± 2.83 | 11.6 ± 4.87 |
| 5 | 38.8 ± 5.32 | 0.8 ± 0.80 | 9.4 ± 4.13 |
| 6 | 28.6 ± 4.66 | 0 ± 0 | 10.2 ± 2.06 |
| 7 | 21.8 ± 4.783-150 | 0.8 ± 0.78 | 6.1 ± 2.11 |
Single CD34+lin− cells were plated at 1 cell/well in serum-free medium and monitored over 7 days as described in Materials and Methods. Data represent the means ± SEM of 4 experiments with a total of 122 wells containing a single cell.
P ≤ .05 significantly different from all cytokines for the number of cells tested except SCF 50 and 100 ng/mL, HGF.