Effect of IL-6 on Primary Lymphocytes Activated With Anti-CD3 Antibody
| Groups . | Apoptosis (%) . | Fas (median) . | FasL (%) . |
|---|---|---|---|
| Anti-CD3 | 54.4 ± 5 | 14 ± 1 | 54.5 ± 7 |
| Anti-CD3 + IL-6 (40 ng/mL) | 28.9 ± 34-150 | 14.5 ± 1 | 22.9 ± 44-150 |
| Anti-CD3 + IL-6 (20 ng/mL) | 31.9 ± 44-150 | 15 ± 2 | 31.08 ± 64-150 |
| Anti-CD3 + IL-6 (10 ng/mL) | 36.4 ± 24-150 | 13.2 ± 3 | 38.5 ± 54-150 |
| Anti-CD3 + IL-6 (1 ng/mL) | 53 ± 6 | 15.5 ± 2 | 56 ± 7 |
| Groups . | Apoptosis (%) . | Fas (median) . | FasL (%) . |
|---|---|---|---|
| Anti-CD3 | 54.4 ± 5 | 14 ± 1 | 54.5 ± 7 |
| Anti-CD3 + IL-6 (40 ng/mL) | 28.9 ± 34-150 | 14.5 ± 1 | 22.9 ± 44-150 |
| Anti-CD3 + IL-6 (20 ng/mL) | 31.9 ± 44-150 | 15 ± 2 | 31.08 ± 64-150 |
| Anti-CD3 + IL-6 (10 ng/mL) | 36.4 ± 24-150 | 13.2 ± 3 | 38.5 ± 54-150 |
| Anti-CD3 + IL-6 (1 ng/mL) | 53 ± 6 | 15.5 ± 2 | 56 ± 7 |
Anti-CD3 (1 μg/mL) MoAb was allowed to adhere in 96-well flat-bottom plates as described above. T lymphocytes (2 × 105/well) were cultured for 5 days on coated plates. Results are expressed as the percentage of apoptosis, the median of Fas expression, the percentage of FasL+ cells, and are the mean of three experiments.
P < .01, significant inhibition comparing lines 2, 3, and 4 with line 1.