IL-6 Inhibits AICD in a Dose-Dependent Manner
| Groups . | Apoptosis (%) . | Fas (median) . | FasL (%) . |
|---|---|---|---|
| Control | 3.1 | 10.7 | 3.1 |
| Anti-CD3 | 31 | 35.3 | 45 |
| Anti-CD3 + IL-6 (20 ng/mL) | 14.6 | 15 | 19 |
| Anti-CD3 + IL-6 (10 ng/mL) | 17.02 | 17.3 | 21 |
| Anti-CD3 + IL-6 (5 ng/mL) | 17.1 | 19.6 | 20.5 |
| Anti-CD3 + IL-6 (1 ng/mL) | 20.1 | 25 | 35 |
| Anti-CD3 + IL-6 (.1 ng/mL) | 31 | 30.7 | 40 |
| Groups . | Apoptosis (%) . | Fas (median) . | FasL (%) . |
|---|---|---|---|
| Control | 3.1 | 10.7 | 3.1 |
| Anti-CD3 | 31 | 35.3 | 45 |
| Anti-CD3 + IL-6 (20 ng/mL) | 14.6 | 15 | 19 |
| Anti-CD3 + IL-6 (10 ng/mL) | 17.02 | 17.3 | 21 |
| Anti-CD3 + IL-6 (5 ng/mL) | 17.1 | 19.6 | 20.5 |
| Anti-CD3 + IL-6 (1 ng/mL) | 20.1 | 25 | 35 |
| Anti-CD3 + IL-6 (.1 ng/mL) | 31 | 30.7 | 40 |
3DO cells were cultured on anti-CD3–coated plates in the presence of different concentrations of IL-6. Data are the means of three experiments. The standard errors are omitted for clarity. Apoptosis of PI-stained nuclei, Fas, and FasL expression were evaluated by FACS analysis after 18 hours culture on anti-CD3–coated plates.