Effect of Exogenous IL-2 on Anti-CD3–Primed 3DO Cells
| Groups . | Apoptosis (%) . | Fas (median) . | FasL (%) . |
|---|---|---|---|
| Control | 2 ± 1 | 14 ± 1 | 2.7 ± .3 |
| Anti-CD3 | 17.5 ± .5 | 20 ± 3 | 22.9 ± 4 |
| Anti-CD3 + IL-2 | 32 ± 4* | 28 ± 4* | 31.08 ± 6* |
| Groups . | Apoptosis (%) . | Fas (median) . | FasL (%) . |
|---|---|---|---|
| Control | 2 ± 1 | 14 ± 1 | 2.7 ± .3 |
| Anti-CD3 | 17.5 ± .5 | 20 ± 3 | 22.9 ± 4 |
| Anti-CD3 + IL-2 | 32 ± 4* | 28 ± 4* | 31.08 ± 6* |
3DO cells were cultured in 96-well-plates coated with anti-CD3 MoAb (1 μg/mL), for 18 hours at 37°C. After washing, cells were incubated with IL-2 (500 IU/mL), in the absence of anti-CD3 MoAb, for an additional 18 hours. Apoptosis of PI-stained nuclei, Fas, and FasL expression were evaluated by FACS analysis as described above. Data are means ± SD of three experiments.
P < .01 comparing line 3 with line 2.