Production of Superoxide Anion by Intact Neutrophils
| Condition . | C Day 0 . | P Day 0 . | C Day 4 . | P Day 4 . |
|---|---|---|---|---|
| PMA (200 ng/mL) | 15.2 ± 1.3 | 13.8 ± 0.8 | 13.4 ± 0.7 | 6.8 ± .53-150 |
| fMLP (10−6 mol/L) | 3.6 ± 1.3 | 2.7 ± 0.6 | 4.8 ± 1.4 | 7.3 ± 1.13-151 |
| PAF/fMLP (2 × 10−6 mol/L PAF) | 33.2 ± 7.4 | 31.3 ± 3.6 | 28.2 ± 3.9 | 16.5 ± 0.83-150 |
| Opsonized zymosan (1 mg/mL) | 5.9 ± 0.7 | 5.5 ± 0.4 | 6.2 ± 0.5 | 5.5 ± 0.4 |
| Condition . | C Day 0 . | P Day 0 . | C Day 4 . | P Day 4 . |
|---|---|---|---|---|
| PMA (200 ng/mL) | 15.2 ± 1.3 | 13.8 ± 0.8 | 13.4 ± 0.7 | 6.8 ± .53-150 |
| fMLP (10−6 mol/L) | 3.6 ± 1.3 | 2.7 ± 0.6 | 4.8 ± 1.4 | 7.3 ± 1.13-151 |
| PAF/fMLP (2 × 10−6 mol/L PAF) | 33.2 ± 7.4 | 31.3 ± 3.6 | 28.2 ± 3.9 | 16.5 ± 0.83-150 |
| Opsonized zymosan (1 mg/mL) | 5.9 ± 0.7 | 5.5 ± 0.4 | 6.2 ± 0.5 | 5.5 ± 0.4 |
Intact cell superoxide anion production was measured as described in Materials and Methods. PMN on day 4 showed reduced NADPH oxidase activity in response to PMA compared with day 0 P ≤ .0001. PMN respiratory burst was increased on day 4 compared with day 0 in response to fMLP (P = .004), while PAF priming of the fMLP response was reduced on day 4 (P ≤ .0001). Superoxide anion production with opsonized zymosan was unaffected by G-CSF therapy. Results represent nmol superoxide anion/min and are expressed as mean ± SEM, n = 8 (matched pairs).
P ≤ .0001.
P ≤ .004.