Cytoplasmic Free Calcium [Ca2+]c Levels in Resting and Stimulated Neutrophils
| . | C Day 0 . | P Day 0 . | C Day 4 . | P Day 4 . |
|---|---|---|---|---|
| fMLP (baseline) | 0.03 ± 0.00 | 0.06 ± 0.01 | 0.04 ± 0.01 | 0.07 ± 0.01 |
| fMLP (peak) | 0.75 ± 0.06 | 0.99 ± 0.07 | 0.99 ± 0.17 | 2.35 ± 0.24*,† |
| PAF (baseline) | 0.07 ± 0.03 | 0.06 ± 0.00 | 0.04 ± 0.00 | 0.11 ± 0.03 |
| PAF (peak) | 1.43 ± 0.32 | 1.55 ± 0.20 | 1.80 ± 0.36 | 3.03 ± 0.65*,† |
| . | C Day 0 . | P Day 0 . | C Day 4 . | P Day 4 . |
|---|---|---|---|---|
| fMLP (baseline) | 0.03 ± 0.00 | 0.06 ± 0.01 | 0.04 ± 0.01 | 0.07 ± 0.01 |
| fMLP (peak) | 0.75 ± 0.06 | 0.99 ± 0.07 | 0.99 ± 0.17 | 2.35 ± 0.24*,† |
| PAF (baseline) | 0.07 ± 0.03 | 0.06 ± 0.00 | 0.04 ± 0.00 | 0.11 ± 0.03 |
| PAF (peak) | 1.43 ± 0.32 | 1.55 ± 0.20 | 1.80 ± 0.36 | 3.03 ± 0.65*,† |
Neutrophil [Ca2+] mobilization was assayed as described in Materials and Methods. There was a slight increase in baseline calcium in the PAF stimulus experiments. On day 4 of G-CSF administration, there was significant enhancement of both PAF and fMLP-stimulated peak mobilization compared with treated day 0 levels (P ≤ .0005) and compared with control day 4 levels (P ≤ .003). Results represent μmol/L concentration of cytosolic calcium and are expressed as mean ± SEM, n = 6 (matched pairs).
P ≤ .0005.
P ≤ .003.