Expression of CD11b During Stimulation of Neutrophils
| Condition . | C Day 0 . | P Day 0 . | C Day 4 . | P Day 4 . |
|---|---|---|---|---|
| PMA (200 ng/mL) | 2.72 ± 0.34 | 2.77 ± 0.35 | 2.5 ± 0.43 | 2.36 ± 0.27 |
| fMLP (10−6mol/L) | 3.53 ± 0.61 | 2.61 ± 0.31 | 2.71 ± 0.33 | 2.09 ± 0.23 |
| PAF (2 × 10−6 mol/L) | 5.39 ± 0.77 | 4.15 ± 0.52 | 3.83 ± 0.36 | 3.58 ± 0.07 |
| Condition . | C Day 0 . | P Day 0 . | C Day 4 . | P Day 4 . |
|---|---|---|---|---|
| PMA (200 ng/mL) | 2.72 ± 0.34 | 2.77 ± 0.35 | 2.5 ± 0.43 | 2.36 ± 0.27 |
| fMLP (10−6mol/L) | 3.53 ± 0.61 | 2.61 ± 0.31 | 2.71 ± 0.33 | 2.09 ± 0.23 |
| PAF (2 × 10−6 mol/L) | 5.39 ± 0.77 | 4.15 ± 0.52 | 3.83 ± 0.36 | 3.58 ± 0.07 |
Neutrophil upregulation of CD11b was measured in response to PMA (200 ng/mL), fMLP (1 × 10−6 mol/L) and PAF (2 × 10−6 mol/L) as described in Materials and Methods. There appeared to be a reduced upregulation of CD11b on treated volunteer day 4, but this was not statistically significant for any stimulus. There also appeared a difference between control day 0 and control day 4 for the PAF-stimulated expression of CD11b, but this was not statistically significant. Data represent the ratio of stimulated CD11b expression to baseline CD11b expression (mean channel fluorescence); n = 6 (matched pairs); mean ± SEM.
Abbreviations: C, control; P, treated volunteer.