Characterization of Long-Term Surviving Animals (B10) Reconstituted With Mobilized PB From B10.BR Donors Treated With FL Alone, G-CSF Alone, or FL + G-CSF
| Donor Treatment . | n . | Donor Chimerism* . | B-Cells (%)† . | αβTCR+ T Cells (%)† . | γδTCR+ T Cells (%)† . | NK Cells (%)† . | Granulocytes (%)‡ . | Macrophages (%)‡ . |
|---|---|---|---|---|---|---|---|---|
| FL | 4 | >99% | 57.5 ± 14.6 | 22.1 ± 8.0 | 0.6 ± 0.1 | 2.3 ± 0.7 | 40.4 ± 5.4 | 35.2 ± 7.5 |
| G-CSF | 4 | >99% | 71.3 ± 0.8 | 15.0 ± 7.4 | 0.5 ± 0.1 | 2.0 ± 0.8 | 41.3 ± 10.5 | 61.9 ± 0.8 |
| FL + G-CSF | 6 | >99% | 48.7 ± 5.4 | 24.5 ± 7.8 | 0.7 ± 0.2 | 2.3 ± 0.4 | 24.4 ± 4.8 | 33.4 ± 8.4 |
| Donor Treatment . | n . | Donor Chimerism* . | B-Cells (%)† . | αβTCR+ T Cells (%)† . | γδTCR+ T Cells (%)† . | NK Cells (%)† . | Granulocytes (%)‡ . | Macrophages (%)‡ . |
|---|---|---|---|---|---|---|---|---|
| FL | 4 | >99% | 57.5 ± 14.6 | 22.1 ± 8.0 | 0.6 ± 0.1 | 2.3 ± 0.7 | 40.4 ± 5.4 | 35.2 ± 7.5 |
| G-CSF | 4 | >99% | 71.3 ± 0.8 | 15.0 ± 7.4 | 0.5 ± 0.1 | 2.0 ± 0.8 | 41.3 ± 10.5 | 61.9 ± 0.8 |
| FL + G-CSF | 6 | >99% | 48.7 ± 5.4 | 24.5 ± 7.8 | 0.7 ± 0.2 | 2.3 ± 0.4 | 24.4 ± 4.8 | 33.4 ± 8.4 |
PB was obtained from representative recipients 6 months after reconstitution. PBMC were stained with lineage- and donor-specific MoAbs for three-color flow cytometric analysis.
Level of donor chimerism was calculated on gated lymphocytes with positive staining for donor (H-2Kk) but negative staining for recipient MHC class I (H-2Kb).
The mean percentage (±SD) of PBMC with positive staining for lineage- and donor-specific MoAbs on gated lymphocytes.
The mean percentage (±SD) of PMBC with positive staining for lineage- and donor-specific MoAbs on gated granulocytes.