Time to Engraftment of Mobilized PBMC From B10.BR Mice Into Lethally Irradiated B10 Mice
| PBMC Dose . | Donor Treatment . | Time to Engraftment: Number of Animals With ≥500 PBMC/μL . | Median PBMC Count (range) [PBMC/μL] . | ||||
|---|---|---|---|---|---|---|---|
| Day 10 . | Day 20 . | Day 30 . | Day 10 . | Day 20 . | Day 30 . | ||
| 1 × 106 | FL | 0/3 | 2/3 | 3/3 | <100 | 560 (200-1,300) | 1,520 (750-4,800) |
| FL + G-CSF | 0/3 | 3/3 | 3/3 | <100 | 850 (620-1,080) | 750 (550-1,350) | |
| 2.5 × 106 | FL | 0/3 | 3/3 | 3/3 | <100 | 920 (520-4,480) | 3,500 (1,100-5,000) |
| FL + G-CSF | 0/3 | 2/3 | 3/3 | <100 | 740 (420-1,460) | 1,900 (1,500-5,600) | |
| 5 × 106 | FL | 1/3 | 2/3 | 3/3 | 220 (180-540) | 620 (460-1,480) | 4,000 (2,300-4,960) |
| FL + G-CSF | 3/3 | 3/3 | 3/3 | 900 (800-920) | 1,360 (1,240-3,960) | 5,800 (5,200-7,100) | |
| PBMC Dose . | Donor Treatment . | Time to Engraftment: Number of Animals With ≥500 PBMC/μL . | Median PBMC Count (range) [PBMC/μL] . | ||||
|---|---|---|---|---|---|---|---|
| Day 10 . | Day 20 . | Day 30 . | Day 10 . | Day 20 . | Day 30 . | ||
| 1 × 106 | FL | 0/3 | 2/3 | 3/3 | <100 | 560 (200-1,300) | 1,520 (750-4,800) |
| FL + G-CSF | 0/3 | 3/3 | 3/3 | <100 | 850 (620-1,080) | 750 (550-1,350) | |
| 2.5 × 106 | FL | 0/3 | 3/3 | 3/3 | <100 | 920 (520-4,480) | 3,500 (1,100-5,000) |
| FL + G-CSF | 0/3 | 2/3 | 3/3 | <100 | 740 (420-1,460) | 1,900 (1,500-5,600) | |
| 5 × 106 | FL | 1/3 | 2/3 | 3/3 | 220 (180-540) | 620 (460-1,480) | 4,000 (2,300-4,960) |
| FL + G-CSF | 3/3 | 3/3 | 3/3 | 900 (800-920) | 1,360 (1,240-3,960) | 5,800 (5,200-7,100) | |
PB was obtained from FL- or FL + G-CSF–treated donors on day 10. Recipients were reconstituted with 1 × 106, 2.5 × 106, or 5 × 106 PBMC and individual PBMC counts were performed 10, 20, and 30 days following PBMC infusion (n = 3 per group). The day at which ≥500 PBMC/μL were detectable was defined as time point of engraftment.