HSC and FC Dose Per Kilogram Body Weight Injected Into Lethally Irradiated B10 Mice
| Donor Treatment . | PBMC Dose/Recipient . | Day 7 . | Day 10 . | ||
|---|---|---|---|---|---|
| HSC Dose/kg [×105] . | FC Dose/kg [×105] . | HSC Dose/kg [×105] . | FC Dose/kg [×105] . | ||
| FL | 1 × 106 | 0.06 ± 0.00 | 0.96 ± 0.07 | 0.63 ± 0.02-150 | 2.10 ± 0.07-150 |
| 2.5 × 106 | 0.17 ± 0.00 | 2.50 ± 0.00 | 1.59 ± 0.24-150 | 5.28 ± 0.79-150 | |
| 5 × 106 | 0.34 ± 0.02 | 5.12 ± 0.26 | 2.84 ± 0.25-150 | 9.41 ± 0.83-150 | |
| G-CSF | 1 × 106 | 0.06 ± 0.00 | 1.06 ± 0.07 | 0.18 ± 0.01 | 0.93 ± 0.07 |
| 2.5 × 106 | 0.17 ± 0.01 | 2.75 ± 0.09 | 0.43 ± 0.02 | 2.22 ± 0.09 | |
| 5 × 106 | 0.33 ± 0.04 | 5.43 ± 0.64 | 0.92 ± 0.24 | 4.77 ± 1.23 | |
| FL + G-CSF | 1 × 106 | 0.29 ± 0.01 | 0.88 ± 0.02 | 0.64 ± 0.03-150 | 1.21 ± 0.06-150 |
| 2.5 × 106 | 0.65 ± 0.04-150 | 1.96 ± 0.13-150 | 1.71 ± 0.07-150 | 3.24 ± 0.13-150 | |
| 5 × 106 | 1.27 ± 0.06-150 | 3.80 ± 0.19-150 | 3.38 ± 0.18-150 | 6.42 ± 0.34-150 | |
| Donor Treatment . | PBMC Dose/Recipient . | Day 7 . | Day 10 . | ||
|---|---|---|---|---|---|
| HSC Dose/kg [×105] . | FC Dose/kg [×105] . | HSC Dose/kg [×105] . | FC Dose/kg [×105] . | ||
| FL | 1 × 106 | 0.06 ± 0.00 | 0.96 ± 0.07 | 0.63 ± 0.02-150 | 2.10 ± 0.07-150 |
| 2.5 × 106 | 0.17 ± 0.00 | 2.50 ± 0.00 | 1.59 ± 0.24-150 | 5.28 ± 0.79-150 | |
| 5 × 106 | 0.34 ± 0.02 | 5.12 ± 0.26 | 2.84 ± 0.25-150 | 9.41 ± 0.83-150 | |
| G-CSF | 1 × 106 | 0.06 ± 0.00 | 1.06 ± 0.07 | 0.18 ± 0.01 | 0.93 ± 0.07 |
| 2.5 × 106 | 0.17 ± 0.01 | 2.75 ± 0.09 | 0.43 ± 0.02 | 2.22 ± 0.09 | |
| 5 × 106 | 0.33 ± 0.04 | 5.43 ± 0.64 | 0.92 ± 0.24 | 4.77 ± 1.23 | |
| FL + G-CSF | 1 × 106 | 0.29 ± 0.01 | 0.88 ± 0.02 | 0.64 ± 0.03-150 | 1.21 ± 0.06-150 |
| 2.5 × 106 | 0.65 ± 0.04-150 | 1.96 ± 0.13-150 | 1.71 ± 0.07-150 | 3.24 ± 0.13-150 | |
| 5 × 106 | 1.27 ± 0.06-150 | 3.80 ± 0.19-150 | 3.38 ± 0.18-150 | 6.42 ± 0.34-150 | |
B10.BR donors were treated with FL alone, G-CSF alone, or FL + G-CSF. PB was collected on day 7 or day 10, pooled for each group, and recipients were reconstituted with either 1 × 106, 2.5 × 106, or 5 × 106 PBMC (4 to 7 mice per group). The dose of HSC (lineage−/Sca-1+/c-kit+) and FC (CD8+/αβTCR−/γδTCR−) per kilogram body weight of recipients was calculated based on flow cytometric analysis and is expressed as the mean ± SD.
Doses at which the 30-day survival reached >80%.