RP105 Transmits an Activation Signal
| MoAb (10 μg/mL) . | 3HTdR Uptake (CPM) . |
|---|---|
| None | 401 ± 65 |
| Control MoAb | 411 ± 166 |
| MHR73 | 10,937 ± 2,000 |
| MoAb (10 μg/mL) . | 3HTdR Uptake (CPM) . |
|---|---|
| None | 401 ± 65 |
| Control MoAb | 411 ± 166 |
| MHR73 | 10,937 ± 2,000 |
Enriched B cells were cultured in a 96-well plate (2 × 105/well) for 4 days with an indicated MoAb (10 μg/mL). Cells were pulsed with 1 μCi of [3H]TdR for the last 6 hours. They were then harvested onto glass fiber filters and the incorporated radioactivity was determined on a Beta plate flat-bed liquid scintillation counter (Pharmacia-Wallac, Gaithersburg, MD). The results are presented as a mean ± SD of triplicate wells.