Effect of Cell Cycle Progression Under TPO-SCF-FL Stimulation on the Repopulating Activity of CD34+ Cells Initially Isolated in G0
| Time in Culture . | Cell Cycle Status . | % Chimerism (%CD45+)* . |
|---|---|---|
| 0 h | G0 | 12.9 |
| 36 h | G0 | 7.2 |
| G1 | 0.5; 0.9 |
| Time in Culture . | Cell Cycle Status . | % Chimerism (%CD45+)* . |
|---|---|---|
| 0 h | G0 | 12.9 |
| 36 h | G0 | 7.2 |
| G1 | 0.5; 0.9 |
NOD/SCID mice were transplanted with 0.7 × 106freshly isolated (0 h) G0CD34+ cells or CD34+ cells initially isolated in G0 and either remaining in G0 or progressing into G1 after 36 hours of ex vivo culture under TPO-SCF-FL stimulation, as shown in Fig.5.
Percentage of CD45+ cells are indicated for each recipient mouse 6 weeks posttransplantation.