Phenotype of Chimeric Human Cells Harvested From NOD/SCID Mice Transplanted With G0CD34+Cells
. | % Engrafted Human Cells With Indicated Phenotype . | ||||||
---|---|---|---|---|---|---|---|
% CD45+Cells . | % CD34+ . | ||||||
CD34+ . | CD19+ . | CD33+ . | CD15+ . | CD38− . | CD19+ . | CD33+ . | |
Mean ± SEM | 19.9 ± 3.1 | 55.7 ± 5.1 | 34.2 ± 5.1 | 35.0 ± 5.2 | 2.1 ± 0.6 | 64.6 ± 2.3 | 18.2 ± 2.4 |
Range | 11.7-29.5 | 34.3-75.9 | 17.2-54.6 | 16.7-53.1 | 0.5-4.8 | 54.1-74.0 | 12.1-27.9 |
. | % Engrafted Human Cells With Indicated Phenotype . | ||||||
---|---|---|---|---|---|---|---|
% CD45+Cells . | % CD34+ . | ||||||
CD34+ . | CD19+ . | CD33+ . | CD15+ . | CD38− . | CD19+ . | CD33+ . | |
Mean ± SEM | 19.9 ± 3.1 | 55.7 ± 5.1 | 34.2 ± 5.1 | 35.0 ± 5.2 | 2.1 ± 0.6 | 64.6 ± 2.3 | 18.2 ± 2.4 |
Range | 11.7-29.5 | 34.3-75.9 | 17.2-54.6 | 16.7-53.1 | 0.5-4.8 | 54.1-74.0 | 12.1-27.9 |
The phenotypic profile of BM cells harvested from NOD/SCID mice transplanted 6 weeks previously with G0CD34+cells was determined by FACScan analysis. Cells were stained with antihuman CD45-FITC or antihuman CD34-FITC and PE-conjugated antihuman MoAbs of indicated specificity. Data shown are from three experiments (n = 2 or 3 animals per experiment).