Expression of IL-2R Chains and Specific mRNA by PBMCs of Healthy Adult Donors: Summary of our Data Presented inBLOOD
| . | CD4 T Lymphocytes . | B Lymphocytes . | CD8 T Lymphocytes . | Monocytes . | NK Cells . |
|---|---|---|---|---|---|
| Cell surface expression (flow cytometry)* | |||||
| α | <5%† | <5% | <5% | <5% | <5% |
| β | <5% | <5% | 14% ± 5.2%‡ | <5% | 78% ± 3.1% |
| γ | <5% | <5% | 8.6% ± 7.5%‡ | 49% ± 6.0%2-153 | <5% |
| Intracellular protein (intracellular flow cytometry) | |||||
| α | <5% | <5% | <5% | ND | <5% |
| β | <5% | <5% | <5% | ND | >40% |
| γ | >60% | >45% | >45% | ND | >60% |
| mRNA (RT-PCR) | |||||
| α | — | — | — | — | — |
| β | — | — | —(+)2-155 | — | + |
| γ | + | + | + | + | + |
| . | CD4 T Lymphocytes . | B Lymphocytes . | CD8 T Lymphocytes . | Monocytes . | NK Cells . |
|---|---|---|---|---|---|
| Cell surface expression (flow cytometry)* | |||||
| α | <5%† | <5% | <5% | <5% | <5% |
| β | <5% | <5% | 14% ± 5.2%‡ | <5% | 78% ± 3.1% |
| γ | <5% | <5% | 8.6% ± 7.5%‡ | 49% ± 6.0%2-153 | <5% |
| Intracellular protein (intracellular flow cytometry) | |||||
| α | <5% | <5% | <5% | ND | <5% |
| β | <5% | <5% | <5% | ND | >40% |
| γ | >60% | >45% | >45% | ND | >60% |
| mRNA (RT-PCR) | |||||
| α | — | — | — | — | — |
| β | — | — | —(+)2-155 | — | + |
| γ | + | + | + | + | + |
Values in bold and underlined are results in disagreement with the letter of Vanham et al, whereas values in italic and underlined are results in partial agreement with the letter of Vanham et al.
Abbreviations: ND, not determined; —, not detected.
Percentage of positive cells.
Expression of less than 5% is considered nonsignificant.
Two kinds of donors can be distinguished. Most of them do not express significantly β or γ IL-2R chains, whereas 30% of them highly expressed these chains. This results in an important standard deviation.
IL-2Rγ chain is expressed for all donors but with a variable intensity.
mRNA detected for few donors in agreement with the ‡ note.