Immunofluorescence Flow Cytometric Analysis of MSCV-FL Leukemic Cells for Coexpression of B-Lymphoid and Myeloid Cell Surface Antigens
| . | Cell Surface Antigen . | ||
|---|---|---|---|
| B220 . | Mac-1 . | B220/Mac-1 . | |
| Leukemic cells | |||
| SPL196 (4°) | 78 | 3.8 | 2.4 |
| SPL196 (3°, cultured) | 19 | 10 | 1.3 |
| SPL199 (5°) | 42 | 15 | 14 |
| SPL199 (4°, cultured) | 6.5 | 74 | 2.1 |
| SPL203 (3°) | 17 | 30 | 12 |
| SPL203 (2°, cultured) | 0.6 | 57 | 0.8 |
| SPL204 (3°) | 86 | 1.8 | 7.8 |
| SPL204 (2°, cultured) | 4.5 | 2.3 | 93 |
| BALB/c SPL | 32 | 5.1 | 2.3 |
| . | Cell Surface Antigen . | ||
|---|---|---|---|
| B220 . | Mac-1 . | B220/Mac-1 . | |
| Leukemic cells | |||
| SPL196 (4°) | 78 | 3.8 | 2.4 |
| SPL196 (3°, cultured) | 19 | 10 | 1.3 |
| SPL199 (5°) | 42 | 15 | 14 |
| SPL199 (4°, cultured) | 6.5 | 74 | 2.1 |
| SPL203 (3°) | 17 | 30 | 12 |
| SPL203 (2°, cultured) | 0.6 | 57 | 0.8 |
| SPL204 (3°) | 86 | 1.8 | 7.8 |
| SPL204 (2°, cultured) | 4.5 | 2.3 | 93 |
| BALB/c SPL | 32 | 5.1 | 2.3 |
Results are the percentages of labeled leukemic spleen (SPL) cells determined by direct staining of FcγRII/III-blocked cells with phycoerythrin-conjugated anti-B220 and fluorescein isothiocyanate-conjugated anti–Mac-1 monoclonal antibodies (as described in Materials and Methods); ≤ 2%, background staining. Spleen cells (5 × 106) were serially transplanted by intravenous injection into recipients that had received 4 Gy of γ-irradiation. Two weeks posttransplantation, single-cell suspensions were prepared from enlarged spleens and analyzed directly or transferred to liquid culture and maintained in the presence of KL, IL-3, and IL-7 plus G418 for 2 to 3 weeks before analysis.