Number of CD34-Enriched Cells Infused, Hematopoietic Recovery, and Survival in Baboons Transplanted With Genetically Marked CD34+ Cells
| Baboon No. . | No. of CD34Enriched Cells/kg ×106 Before Culture (% CD34+) . | Transduction Conditions for Indicated Vector . | No. of CD34Enriched Cells Infused/kg ×106 (LNX/LN) (total) . | ANC-150 >500/μL . | Survival in Months (cause of death) . | |
|---|---|---|---|---|---|---|
| LNX . | LN . | |||||
| T95096 | 4.8 (94%) | 48-h cocultivation, IL-3, IL-6, SCF, PS | 48-h CH-296, IL-3, IL-6, SCF | 1.9/2.0 (3.9) | 12 | >13 |
| F94424 | 4.6 (97%) | 48-h cocultivation, IL-3, IL-6, SCF, PS | 48-h CH-296, IL-3, IL-6, SCF | 0.45/1.8 (2.25) | 11 | 1 (sepsis) |
| T94397 | 5.7 (83%) | 48-h cocultivation, IL-3, IL-6, SCF, PS | 48-h CH-296, IL-3, IL-6, SCF, PS | 0.7/1.3 (2.0) | 20 | >10 |
| J95009 | 7.5 (83%) | 72-h cocultivation, IL-3, IL-6, SCF, PS | 72-h CH-296, IL-6, SCF, FLT3L, MGDF, PS | 3.7/4.4 (8.1) | 14 | >9 |
| T94433 | 8.4 (90%) | 72-h cocultivation, IL-6, SCF, FLT3L, MGDF, PS | 72-h CH-296, IL-6, SCF, FLT3L, MGDF, PS | 4.5/5.5 (10) | 13 | >8 |
| M94123 | 7.0 (90%) | 72-h BSA, IL-6, SCF, FLT3L, MGDF, PS | 72-h CH-296, IL-6, SCF, FLT3L, MGDF, PS | 5.3/4.7 (10) | 11 | >6 |
| Baboon No. . | No. of CD34Enriched Cells/kg ×106 Before Culture (% CD34+) . | Transduction Conditions for Indicated Vector . | No. of CD34Enriched Cells Infused/kg ×106 (LNX/LN) (total) . | ANC-150 >500/μL . | Survival in Months (cause of death) . | |
|---|---|---|---|---|---|---|
| LNX . | LN . | |||||
| T95096 | 4.8 (94%) | 48-h cocultivation, IL-3, IL-6, SCF, PS | 48-h CH-296, IL-3, IL-6, SCF | 1.9/2.0 (3.9) | 12 | >13 |
| F94424 | 4.6 (97%) | 48-h cocultivation, IL-3, IL-6, SCF, PS | 48-h CH-296, IL-3, IL-6, SCF | 0.45/1.8 (2.25) | 11 | 1 (sepsis) |
| T94397 | 5.7 (83%) | 48-h cocultivation, IL-3, IL-6, SCF, PS | 48-h CH-296, IL-3, IL-6, SCF, PS | 0.7/1.3 (2.0) | 20 | >10 |
| J95009 | 7.5 (83%) | 72-h cocultivation, IL-3, IL-6, SCF, PS | 72-h CH-296, IL-6, SCF, FLT3L, MGDF, PS | 3.7/4.4 (8.1) | 14 | >9 |
| T94433 | 8.4 (90%) | 72-h cocultivation, IL-6, SCF, FLT3L, MGDF, PS | 72-h CH-296, IL-6, SCF, FLT3L, MGDF, PS | 4.5/5.5 (10) | 13 | >8 |
| M94123 | 7.0 (90%) | 72-h BSA, IL-6, SCF, FLT3L, MGDF, PS | 72-h CH-296, IL-6, SCF, FLT3L, MGDF, PS | 5.3/4.7 (10) | 11 | >6 |
Abbreviations: ANC, absolute neutrophil count; BSA, bovine serum albumin; PS, protamine sulfate.
Day to reach a sustained neutrophil count greater than 500/μL.