Phenotypic Analysis by Immunofluorescent Staining of Colony Progeny
| 11 dpc: . | YS . | FBL . | FL . | ||||||
|---|---|---|---|---|---|---|---|---|---|
| SCF . | Eotaxin . | Both . | SCF . | Eotaxin . | Both . | SCF . | Eotaxin . | Both . | |
| Experiment 1 | |||||||||
| Mac-1 | 12.7 | 12.7 | 15.2 | 2.12 | 6.9 | 14.8 | 17.4 | 24.8 | 34.3 |
| TER-119 | 6.4 | 1.7 | <1.0 | 3.2 | 5.7 | 2.7 | 3.4 | 2.3 | 2.5 |
| Gr-1 | 2.1 | 1.0 | <1.0 | 0 | 3.8 | 5.7 | 6.7 | 4.7 | 3.0 |
| Experiment 2 | |||||||||
| Mac-1 | 7.7 | 8.3 | 34.2 | 6.8 | 5.8 | 22.0 | 10.5 | 32.4 | 47.2 |
| TER-119 | 0 | 0 | 1.9 | 4.6 | 3.6 | 2.7 | 6.1 | 14.7 | <1.0 |
| Gr-1 | 0 | 1.3 | 1.8 | 15.4 | 15.5 | 15.9 | 8.7 | 8.1 | 3.5 |
| VLA-4 | 16.3 | 8.6 | 6.2-150 | 5.0 | 5.8 | 5.0 | — | — | — |
| Experiment 3 | |||||||||
| c-kit | — | — | 21.0 | — | — | 12.9 | — | — | — |
| B220 | — | — | 4.0 | — | — | 2.5 | — | — | — |
| Thy-1 | — | — | 0 | — | — | 6.6 | — | — | — |
| 11 dpc: . | YS . | FBL . | FL . | ||||||
|---|---|---|---|---|---|---|---|---|---|
| SCF . | Eotaxin . | Both . | SCF . | Eotaxin . | Both . | SCF . | Eotaxin . | Both . | |
| Experiment 1 | |||||||||
| Mac-1 | 12.7 | 12.7 | 15.2 | 2.12 | 6.9 | 14.8 | 17.4 | 24.8 | 34.3 |
| TER-119 | 6.4 | 1.7 | <1.0 | 3.2 | 5.7 | 2.7 | 3.4 | 2.3 | 2.5 |
| Gr-1 | 2.1 | 1.0 | <1.0 | 0 | 3.8 | 5.7 | 6.7 | 4.7 | 3.0 |
| Experiment 2 | |||||||||
| Mac-1 | 7.7 | 8.3 | 34.2 | 6.8 | 5.8 | 22.0 | 10.5 | 32.4 | 47.2 |
| TER-119 | 0 | 0 | 1.9 | 4.6 | 3.6 | 2.7 | 6.1 | 14.7 | <1.0 |
| Gr-1 | 0 | 1.3 | 1.8 | 15.4 | 15.5 | 15.9 | 8.7 | 8.1 | 3.5 |
| VLA-4 | 16.3 | 8.6 | 6.2-150 | 5.0 | 5.8 | 5.0 | — | — | — |
| Experiment 3 | |||||||||
| c-kit | — | — | 21.0 | — | — | 12.9 | — | — | — |
| B220 | — | — | 4.0 | — | — | 2.5 | — | — | — |
| Thy-1 | — | — | 0 | — | — | 6.6 | — | — | — |
The numbers shown are the percentage of cells positive for each lineage marker (minus background staining) after 10 days in culture. A (—) indicates that cells were not tested.
28.7% of YS progeny were positive for VLA-4 when grown in the presence of SCF, eotaxin, and pertussis toxin.