Synthetic Oligonucleotides for Analysis of Rh50 Glycoprotein Gene
| Category* . | Sequence . | nt Position-151 . | Location . |
|---|---|---|---|
| I. cDNA PCR | |||
| 3′-UTa | 5′-AATGGGAAAGGAAGCTGGAGAGCA-3′ | 1321-1298 | 3′-UTR |
| Ex-1s | 5′-AGTGTGCCTCTGTCCTTTGCCACA-3′ | −27-−4 | Exon 1 |
| Ex-4s | 5′-GAAGAGTCCGCATACTACTCAGAC-3′ | 601-624 | Exon 4 |
| Ex-5a | 5′-CTGTTTGTCTCCAGGTTCAGCAAT-3′ | 708-605 | Exon 5 |
| Ex-10a | 5′-CCATGTCCATGGAACTGATTGTCA-3′ | 1256-1233 | Exon 10 |
| II. Genomic PCR | |||
| 5′P2 | 5′-TCCAGCTTTGCTTGACTGATTGCC-3′ | −679-−656 | 5′ region |
| 5′P3 | 5′-CACAGGCTGTGAGATGTTGATAAG-3′ | −36-−54 | 5′ region |
| 1Ps | 5′-AGAACGACAATTAGATTACAGAGC-3′ | −202-−179 | 5′ region |
| In-1a | 5′-CAAAGAGCTCAAGGGTTTATAGTA-3′ | 72-95 | Intron 1 |
| In-1s | 5′-TCCTAGTGTGTTATACAAAGGTGG-3′ | 111-88 | Intron 1 |
| In-2a | 5′-AACACTCTGGCTTCATGACCTGGT-3′ | 117-130 | Intron 2 |
| In-2s | 5′-TGGTGGGAGATGGGATTGCACTTT-3′ | 57-80 | Intron 2 |
| In-3a | 5′-CAAGATTTGCTCCCATATACCGTA-3′ | 72-95 | Intron 3 |
| In-3s | 5′-ATTGACTTTCCTCCCATAAATGAG-3′ | 46-69 | Intron 3 |
| In-4a | 5′-CTGGCTTGGATGTTCTTTTTGAGC-3′ | 81-104 | Intron 4 |
| In-4s | 5′-TGACAGAGGGACACTCCATCTCAA-3′ | 101-124 | Intron 4 |
| In-5a | 5′-CAGCAGTGATGCAGAAATTACCTA-3′ | 67-90 | Intron 5 |
| In-5s | 5′-TTTCAACTGAAGAATGTGATGTGG-3′ | 85-107 | Intron 5 |
| In-6a | 5′-CCAAAAAGAAGGCAAATGGTCTCC-3′ | 111-134 | Intron 6 |
| Ex-6a | 5′-AGTCAGGAACTTGTATCCAAGCAC-3′ | 945-922 | Exon 6 |
| In-6s | 5′-GCCCAGCTATAGCTGTGTTTCAGT-3′ | 57-80 | Intron 6 |
| In-7a | 5′-CTAATGATCTTCTCTCAGGCGCGT-3′ | 129-152 | Intron 7 |
| In-7s | 5′-ATGTTACACTCTGGCCTGAAGATG-3′ | 21-44 | Intron 7 |
| In-8a | 5′-ACTGCCAGAAGTTTAGAACATTTT-3′ | 91-114 | Intron 8 |
| In-8s | 5′-TGTTCTAAACTTCTGGCAGTAAAG-3′ | 111-134 | Intron 8 |
| In-9a | 5′-CCTACCATGCCAGGCTAACTCAAT-3′ | 101-124 | Intron 9 |
| In-9s | 5′-TCCCAGTTCTTTCCAAGCTTGAAT-3′ | 95-118 | Intron 9 |
| 3′-UTa | 5′-AATGGGAAAGGAAGCTGGAGAGCA-3′ | 1321-1298 | 3′ region |
| Category* . | Sequence . | nt Position-151 . | Location . |
|---|---|---|---|
| I. cDNA PCR | |||
| 3′-UTa | 5′-AATGGGAAAGGAAGCTGGAGAGCA-3′ | 1321-1298 | 3′-UTR |
| Ex-1s | 5′-AGTGTGCCTCTGTCCTTTGCCACA-3′ | −27-−4 | Exon 1 |
| Ex-4s | 5′-GAAGAGTCCGCATACTACTCAGAC-3′ | 601-624 | Exon 4 |
| Ex-5a | 5′-CTGTTTGTCTCCAGGTTCAGCAAT-3′ | 708-605 | Exon 5 |
| Ex-10a | 5′-CCATGTCCATGGAACTGATTGTCA-3′ | 1256-1233 | Exon 10 |
| II. Genomic PCR | |||
| 5′P2 | 5′-TCCAGCTTTGCTTGACTGATTGCC-3′ | −679-−656 | 5′ region |
| 5′P3 | 5′-CACAGGCTGTGAGATGTTGATAAG-3′ | −36-−54 | 5′ region |
| 1Ps | 5′-AGAACGACAATTAGATTACAGAGC-3′ | −202-−179 | 5′ region |
| In-1a | 5′-CAAAGAGCTCAAGGGTTTATAGTA-3′ | 72-95 | Intron 1 |
| In-1s | 5′-TCCTAGTGTGTTATACAAAGGTGG-3′ | 111-88 | Intron 1 |
| In-2a | 5′-AACACTCTGGCTTCATGACCTGGT-3′ | 117-130 | Intron 2 |
| In-2s | 5′-TGGTGGGAGATGGGATTGCACTTT-3′ | 57-80 | Intron 2 |
| In-3a | 5′-CAAGATTTGCTCCCATATACCGTA-3′ | 72-95 | Intron 3 |
| In-3s | 5′-ATTGACTTTCCTCCCATAAATGAG-3′ | 46-69 | Intron 3 |
| In-4a | 5′-CTGGCTTGGATGTTCTTTTTGAGC-3′ | 81-104 | Intron 4 |
| In-4s | 5′-TGACAGAGGGACACTCCATCTCAA-3′ | 101-124 | Intron 4 |
| In-5a | 5′-CAGCAGTGATGCAGAAATTACCTA-3′ | 67-90 | Intron 5 |
| In-5s | 5′-TTTCAACTGAAGAATGTGATGTGG-3′ | 85-107 | Intron 5 |
| In-6a | 5′-CCAAAAAGAAGGCAAATGGTCTCC-3′ | 111-134 | Intron 6 |
| Ex-6a | 5′-AGTCAGGAACTTGTATCCAAGCAC-3′ | 945-922 | Exon 6 |
| In-6s | 5′-GCCCAGCTATAGCTGTGTTTCAGT-3′ | 57-80 | Intron 6 |
| In-7a | 5′-CTAATGATCTTCTCTCAGGCGCGT-3′ | 129-152 | Intron 7 |
| In-7s | 5′-ATGTTACACTCTGGCCTGAAGATG-3′ | 21-44 | Intron 7 |
| In-8a | 5′-ACTGCCAGAAGTTTAGAACATTTT-3′ | 91-114 | Intron 8 |
| In-8s | 5′-TGTTCTAAACTTCTGGCAGTAAAG-3′ | 111-134 | Intron 8 |
| In-9a | 5′-CCTACCATGCCAGGCTAACTCAAT-3′ | 101-124 | Intron 9 |
| In-9s | 5′-TCCCAGTTCTTTCCAAGCTTGAAT-3′ | 95-118 | Intron 9 |
| 3′-UTa | 5′-AATGGGAAAGGAAGCTGGAGAGCA-3′ | 1321-1298 | 3′ region |
*Sense (s) and antisense (a) primers to 3′-UT, exon (Ex), 5′-region (P) and intron (In) are denoted.
Nucleotide positions of exon primers are accounted from the first base of initiation codon ATG, whereas intron primers are designated by their distance from the exons they encompass.