NAD+ Hydrolysis Activities in CD38+/+ and CD38−/− Mice
| . | NAD+Glycohydrolase Activity-150 Vm (nmoles/min/mg protein) . | Nucleotide Pyrophosphatase Activity-150 Vm (nmoles/min/mg protein) . | ||
|---|---|---|---|---|
| CD38+/+ . | CD38−/− . | CD38+/+ . | CD38−/− . | |
| Spleen (n = 3) | 87.2 ± 4.1 | 0.59 ± 0.15 | 0.34 ± 0.1 | 0.33 ± 0.14 |
| Liver (n = 3) | 19.3 ± 1.6 | ND | 0.63 ± 0.23 | 0.63 ± 0.20 |
| Brain (n = 3) | 22.5 ± 1.7 | ND | 0.49 ± 0.19 | 0.46 ± 0.19 |
| . | NAD+Glycohydrolase Activity-150 Vm (nmoles/min/mg protein) . | Nucleotide Pyrophosphatase Activity-150 Vm (nmoles/min/mg protein) . | ||
|---|---|---|---|---|
| CD38+/+ . | CD38−/− . | CD38+/+ . | CD38−/− . | |
| Spleen (n = 3) | 87.2 ± 4.1 | 0.59 ± 0.15 | 0.34 ± 0.1 | 0.33 ± 0.14 |
| Liver (n = 3) | 19.3 ± 1.6 | ND | 0.63 ± 0.23 | 0.63 ± 0.20 |
| Brain (n = 3) | 22.5 ± 1.7 | ND | 0.49 ± 0.19 | 0.46 ± 0.19 |
NAD+ glycohydrolase and nucleotide pyrophosphatase activity measurements were made on tissue homogenates prepared from individual 12-week-old 129Ola × C57BL/6J F3 CD38−/−mice and CD38+/+ littermate controls. Data shown represent the mean ± standard deviation of three individual mice per determination.
Abbreviation: ND, not detected.
NAD+ glycohydrolase and nucleotide pyrophosphatase activity measurements were determined by fluorometric enzyme assays as described in the Materials and Methods.