Recovery of Steady-State BM and Mobilized PB CD34+ Cells After Coculture on MS-5 Monolayer
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Recovery of CD34+Cells (%)
.
Nonadherent Fraction
.
Adherent Fraction
.
Steady-state BM (N = 6)
65 ± 1.4
32 ± 2.2
Mobilized PB (N = 4)
83 ± 2.1
17 ± 3.4
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Recovery of CD34+Cells (%)
.
Nonadherent Fraction
.
Adherent Fraction
.
Steady-state BM (N = 6)
65 ± 1.4
32 ± 2.2
Mobilized PB (N = 4)
83 ± 2.1
17 ± 3.4
1.0 × 105 steady-state BM and mobilized PB CD34+ cells were cultured on a monolayer of MS-5 cells. After a 1-hour incubation nonadherent cells were recovered by gentle agitation and then adherent cells by vigorous pipetting.