Table 2.

Recovery of Steady-State BM and Mobilized PB CD34+ Cells After Coculture on MS-5 Monolayer

Recovery of CD34+Cells (%)
Nonadherent Fraction Adherent Fraction
Steady-state BM (N = 6) 65 ± 1.4 32 ± 2.2  
Mobilized PB (N = 4)  83 ± 2.1  17 ± 3.4 
Recovery of CD34+Cells (%)
Nonadherent Fraction Adherent Fraction
Steady-state BM (N = 6) 65 ± 1.4 32 ± 2.2  
Mobilized PB (N = 4)  83 ± 2.1  17 ± 3.4 

1.0 × 105 steady-state BM and mobilized PB CD34+ cells were cultured on a monolayer of MS-5 cells. After a 1-hour incubation nonadherent cells were recovered by gentle agitation and then adherent cells by vigorous pipetting.

*

P < .001 by multiple t-test.

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