Flow Cytometry Analyses
| (A) Flow Cytometry Analysis in Children With PAP Before Therapy . | ||||||
|---|---|---|---|---|---|---|
| Patient No. . | Age at Diagnosis . | AML . | βc . | GM-CSFR− α . | IL-3R− α . | Material . |
| 1 | 0.5 mo | M4 | 5% | 90% | 25% | PB |
| <1% | 18% | 10% | BAL | |||
| 2 | 11 yr | M0 | <1% | <1% | 39% | BM |
| <1% | <1% | 8% | BAL | |||
| 3 | 9 mo | M5 | <1% | <1% | 27% | BM(th) |
| ND | ND | ND | BAL |
| (A) Flow Cytometry Analysis in Children With PAP Before Therapy . | ||||||
|---|---|---|---|---|---|---|
| Patient No. . | Age at Diagnosis . | AML . | βc . | GM-CSFR− α . | IL-3R− α . | Material . |
| 1 | 0.5 mo | M4 | 5% | 90% | 25% | PB |
| <1% | 18% | 10% | BAL | |||
| 2 | 11 yr | M0 | <1% | <1% | 39% | BM |
| <1% | <1% | 8% | BAL | |||
| 3 | 9 mo | M5 | <1% | <1% | 27% | BM(th) |
| ND | ND | ND | BAL |
| (B) Flow Cytometry Analysis in Controls . | |||||
|---|---|---|---|---|---|
| Healthy Controls . | Age . | βc . | GM-CSFR α . | IL-3R− α . | Material . |
| 1 | 1 mo | 85% | 88% | 34% | PB |
| 2 | 4 yr | 86% | 90% | 9% | BM(th) |
| 3 | 12 yr | 83% | 88% | 22% | BM |
| (B) Flow Cytometry Analysis in Controls . | |||||
|---|---|---|---|---|---|
| Healthy Controls . | Age . | βc . | GM-CSFR α . | IL-3R− α . | Material . |
| 1 | 1 mo | 85% | 88% | 34% | PB |
| 2 | 4 yr | 86% | 90% | 9% | BM(th) |
| 3 | 12 yr | 83% | 88% | 22% | BM |
| Control AML Patients . | Age at Diagnosis . | AML . | βc . | GM-CSFR α . | IL-3R− α . | Material . |
|---|---|---|---|---|---|---|
| 1 | 1 mo | M0 | 85% | 75% | 11% | BM |
| 2 | 42 yr | M1 | 86% | 67% | 20% | BM |
| 3 | 40 yr | M1 | 75% | 85% | 54% | BM |
| 4 | 27 yr | M1 | 95% | 92% | 19% | BM |
| 5 | 66 yr | M2 | 61% | 77% | 12% | BM |
| 6 | 11 yr | M2 | 61% | 80% | 35% | BM |
| 7 | 53 yr | M2 | 92% | 78% | 18% | BM |
| 8 | 54 yr | M4 | 67% | 65% | 23% | BM |
| 9 | 63 yr | M4 | 53% | 54% | 55% | BM |
| 10 | 69 yr | M4 | 62% | 87% | 42% | BM |
| 11 | 54 yr | M4 | 65% | 64% | 16% | BM |
| 12 | 28 yr | M4 | 94% | 92% | 65% | BM |
| 13 | 63 yr | M4 | 96% | 67% | 44% | BM |
| 14 | 25 yr | M5 | 95% | 95% | 10% | BM |
| 15 | 35 yr | M5 | 48% | 95% | 32% | BM |
| 16 | 52 yr | M5 | 60% | 70% | 35% | BM |
| 17 | 4 yr | RAEB | 92% | 63% | 12% | BM |
| Control AML Patients . | Age at Diagnosis . | AML . | βc . | GM-CSFR α . | IL-3R− α . | Material . |
|---|---|---|---|---|---|---|
| 1 | 1 mo | M0 | 85% | 75% | 11% | BM |
| 2 | 42 yr | M1 | 86% | 67% | 20% | BM |
| 3 | 40 yr | M1 | 75% | 85% | 54% | BM |
| 4 | 27 yr | M1 | 95% | 92% | 19% | BM |
| 5 | 66 yr | M2 | 61% | 77% | 12% | BM |
| 6 | 11 yr | M2 | 61% | 80% | 35% | BM |
| 7 | 53 yr | M2 | 92% | 78% | 18% | BM |
| 8 | 54 yr | M4 | 67% | 65% | 23% | BM |
| 9 | 63 yr | M4 | 53% | 54% | 55% | BM |
| 10 | 69 yr | M4 | 62% | 87% | 42% | BM |
| 11 | 54 yr | M4 | 65% | 64% | 16% | BM |
| 12 | 28 yr | M4 | 94% | 92% | 65% | BM |
| 13 | 63 yr | M4 | 96% | 67% | 44% | BM |
| 14 | 25 yr | M5 | 95% | 95% | 10% | BM |
| 15 | 35 yr | M5 | 48% | 95% | 32% | BM |
| 16 | 52 yr | M5 | 60% | 70% | 35% | BM |
| 17 | 4 yr | RAEB | 92% | 63% | 12% | BM |
(A) Flow cytometry analysis of the GM-CSF/IL-3/IL-5 receptors’ common β chain (βc), GM-CSF receptor α chain (GM-CSFR α), and IL-3 receptor α chain (IL-3Rα) in peripheral blood (PB), bone marrow aspirate (BM), or broncheo alveolar lavage fluid (BAL) from 2 patients with AML and PAP and 1 child with AML and severe lung disease. Data are expressed as the percentage of cells in the myeloid gate. The AML classification was performed according to FAB.
(B) Flow cytometry analysis of the GM-CSF/IL-3/IL-5 receptors common β chain (βc), GM-CSF receptor α chain (GM-CSFR α), and IL-3 receptor α chain (IL-3Rα) in peripheral blood (PB), bone marrow aspirate (BM), or broncheo alveolar lavage fluid (BAL) from 16 patients with AML without PAP, 1 refractory anemia with blast excess (RAEB), 3 age-matched controls, and 1 control BAL from a child after peanut aspiration. Data are expressed as the percentage of cells in the myeloid gate. The AML classification was performed according to FAB.
Abbreviations: ND, not determined; th, thawed.