Oligonucletide Primers for the Amplification and Detection of Interleukins and Receptors
| Molecule . | Sequence Reference . | Primer . | Sequence (5′ to 3′) . | Position . | Predicted Size . |
|---|---|---|---|---|---|
| IL-1α | Lomedico32 | Forward | CAAACTGATGAAGCTCGTCA | 438-457 | 225 bp |
| Backward | TCTCCTTGAGCGCTCACGAA | 643-662 | |||
| IL-1β | Gray33 | Forward | TCATGGGATGATGATGATAACCTGC | 393-418 | 503 bp |
| Backward | CCCATACTTTAGGAAGACACGGATT | 871-895 | |||
| IL-2 | Yokota34 | Forward | TGAGCAGGATGGAGAAT | 224-240 | 360 bp |
| Backward | TGTGTTGTAAGCAGGAG | 576-583 | |||
| IL-3 | Fung35 | Forward | GAGGACCAGAACGAGACA | 10-27 | 537 bp |
| Backward | GGTGCTCTGCCTGCTGTT | 529-546 | |||
| IL-4 | Sideras36 | Forward | ATGGGTCTCAACCCCCAGCTAGT | 88-110 | 399 bp |
| Backward | GCTCTTTAGGCTTTCCAGCTAGT | 464-486 | |||
| IL-6 | Van Snick37 | Forward | TTGGGACTGATGCTGGTG | 91-108 | 426 bp |
| Backward | CTCTTGGTTGAAGATATGAA | 497-516 | |||
| IL-7 | Lupton38 | Forward | GCCTGTCACATCATCTGAGTGCC | 608-630 | 496 bp |
| Backward | CAGGAGGCATCCAGGAACTTCTG | 1081-1103 | |||
| IL-8 | Tekamp-Olson39 | Forward | CACTCCAGACTCCAGCCA | 12-29 | 719 bp |
| Backward | TCCCAACTCACCCTCTCC | 703-720 | |||
| IL-11 | Paul21 | Forward | ATGAACTGTGTTTGTCGCCTG | 1-21 | 200 bp |
| Backward | TCAGCTGGGAATTTGTCTCTC | 180-200 | |||
| IL-1RI | Sims40 | Forward | CTGAGCCCTCGGAATGAGACG | 637-657 | 702 bp |
| Backward | GCTGAAGCCTCCCATATCTCT | 1318-1338 | |||
| IL-11R | Hilton23 | Forward | TTGCCCACCCGCTACCTTACTTC | 418-440 | 431 bp |
| Backward | GCATCTGTTATCACTTCCTCCAA | 826-848 |
| Molecule . | Sequence Reference . | Primer . | Sequence (5′ to 3′) . | Position . | Predicted Size . |
|---|---|---|---|---|---|
| IL-1α | Lomedico32 | Forward | CAAACTGATGAAGCTCGTCA | 438-457 | 225 bp |
| Backward | TCTCCTTGAGCGCTCACGAA | 643-662 | |||
| IL-1β | Gray33 | Forward | TCATGGGATGATGATGATAACCTGC | 393-418 | 503 bp |
| Backward | CCCATACTTTAGGAAGACACGGATT | 871-895 | |||
| IL-2 | Yokota34 | Forward | TGAGCAGGATGGAGAAT | 224-240 | 360 bp |
| Backward | TGTGTTGTAAGCAGGAG | 576-583 | |||
| IL-3 | Fung35 | Forward | GAGGACCAGAACGAGACA | 10-27 | 537 bp |
| Backward | GGTGCTCTGCCTGCTGTT | 529-546 | |||
| IL-4 | Sideras36 | Forward | ATGGGTCTCAACCCCCAGCTAGT | 88-110 | 399 bp |
| Backward | GCTCTTTAGGCTTTCCAGCTAGT | 464-486 | |||
| IL-6 | Van Snick37 | Forward | TTGGGACTGATGCTGGTG | 91-108 | 426 bp |
| Backward | CTCTTGGTTGAAGATATGAA | 497-516 | |||
| IL-7 | Lupton38 | Forward | GCCTGTCACATCATCTGAGTGCC | 608-630 | 496 bp |
| Backward | CAGGAGGCATCCAGGAACTTCTG | 1081-1103 | |||
| IL-8 | Tekamp-Olson39 | Forward | CACTCCAGACTCCAGCCA | 12-29 | 719 bp |
| Backward | TCCCAACTCACCCTCTCC | 703-720 | |||
| IL-11 | Paul21 | Forward | ATGAACTGTGTTTGTCGCCTG | 1-21 | 200 bp |
| Backward | TCAGCTGGGAATTTGTCTCTC | 180-200 | |||
| IL-1RI | Sims40 | Forward | CTGAGCCCTCGGAATGAGACG | 637-657 | 702 bp |
| Backward | GCTGAAGCCTCCCATATCTCT | 1318-1338 | |||
| IL-11R | Hilton23 | Forward | TTGCCCACCCGCTACCTTACTTC | 418-440 | 431 bp |
| Backward | GCATCTGTTATCACTTCCTCCAA | 826-848 |