Differential Leukocyte Counts in Peripheral Blood of Normal BL6 Mice and BL6 Mice With Homozygous or Heterozygous Deficiency of Class-II MHC Molecules
| . | C57BL/6 Mice . | ||
|---|---|---|---|
| Wild-Type (n = 6)* . | Heterozygous (n = 6)* . | Homozygous (n = 6)* . | |
| WBC Conc. (×10−4 cells/μL) | 1.24 ± 0.22 | 1.13 ± 0.30 | 1.34 ± 0.35 |
| Differential Counts† | |||
| Lymphocytes (%) | 80 ± 6 | 82 ± 6 | 79 ± 8 |
| Monocytes (%) | 4 ± 2 | 4 ± 1 | 4 ± 2 |
| PMN (%) | 16 ± 5 | 13 ± 6 | 17 ± 7 |
| Subsets of Lymphocytes‡ | |||
| B cells (%) | 35 ± 5 | 39 ± 10 | 40 ± 4 |
| CD3+ cells (%) | 46 ± 4 | 38 ± 9 | 40 ± 9 |
| CD4+ cells (%) | 25 ± 4 | 18 ± 3 | 2 ± 1 |
| CD8+ cells (%) | 19 ± 4 | 18 ± 6 | 36 ± 61-153 |
| . | C57BL/6 Mice . | ||
|---|---|---|---|
| Wild-Type (n = 6)* . | Heterozygous (n = 6)* . | Homozygous (n = 6)* . | |
| WBC Conc. (×10−4 cells/μL) | 1.24 ± 0.22 | 1.13 ± 0.30 | 1.34 ± 0.35 |
| Differential Counts† | |||
| Lymphocytes (%) | 80 ± 6 | 82 ± 6 | 79 ± 8 |
| Monocytes (%) | 4 ± 2 | 4 ± 1 | 4 ± 2 |
| PMN (%) | 16 ± 5 | 13 ± 6 | 17 ± 7 |
| Subsets of Lymphocytes‡ | |||
| B cells (%) | 35 ± 5 | 39 ± 10 | 40 ± 4 |
| CD3+ cells (%) | 46 ± 4 | 38 ± 9 | 40 ± 9 |
| CD4+ cells (%) | 25 ± 4 | 18 ± 3 | 2 ± 1 |
| CD8+ cells (%) | 19 ± 4 | 18 ± 6 | 36 ± 61-153 |
*There were six 12-week old mice in each group. Three were female and three were male.
Differential counts were determined by evaluating 250 leukocytes in a peripheral blood smear.
Subsets of lymphocytes were determined by using immunofluorescent flow cytometry. Peripheral blood mononuclear leukocytes were isolated by centrifugation through Ficoll-hypaque. Each value is expressed as % of total mononuclear leukocytes.
The value is significantly higher than those of normal (P = .0001) and heterozygous (P = .0003) BL/6 mice.