Absolute Cell Counts of Total CD8α+ or CD8αβ+ Cells of CD8α+ T Cells, CD8αbright T Cells, NK Cells, TCRγδ+ T Cells, and CD4+ T Cells
| Assessed Cell Subset . | HIV-1 Seronegative (n = 21) . | HIV-1 Seropositive >200 CD4+ cells/μL (n = 58) . | HIV-1 Seropositive <200 CD4+ cells/μL (n = 45) . |
|---|---|---|---|
| Absolute Cell Counts (25%, 75%) . | |||
| CD8α+ T cells | 544 (371, 624)* | 1028 (838, 1389) | 762 (422, 932)* |
| CD8αβ+CD8α+ T cells | 463 (345, 560)* | 981 (784, 1300) | 733 (415, 903)* |
| CD8αbright T cells | 461 (331, 552)* | 942 (768, 1289) | 712 (408, 899)* |
| CD8αβ+CD8αbright T cells | 444 (313, 528) | 934 (752, 1276) | 700 (404, 891) |
| NK cells | 260 (155, 429) | 72 (36, 138)* | 82 (53, 163)* |
| CD8α+ NK cells | 154 (72, 190) | 18 (10, 33)* | 30 (11, 41)* |
| CD8αβ+ NK cells | 4 (3, 8) | 1 (0, 3)* | 1 (0, 2)* |
| TCRγδ+ T cells | 74 (37, 128)† | 90 (53, 141)† | 64 (33, 94)† |
| TCRγδ+CD8α+ T cells | 20 (12, 31)* | 40 (22, 58) | 25 (15, 39)* |
| TCRγδ+CD8αβ+ T cells | 3 (1, 5) | 16 (10, 27) | 10 (5, 13) |
| CD4+ T cells | 857 (779, 965) | 408 (330, 525) | 102 (58, 150) |
| CD4+CD8α+ T cells | 12 (8, 14)* | 14 (8, 29)* | 4 (3, 8) |
| CD4+CD8αβ+ T cells | 5 (3, 7)* | 7 (4, 12)* | 3 (2, 5) |
| Assessed Cell Subset . | HIV-1 Seronegative (n = 21) . | HIV-1 Seropositive >200 CD4+ cells/μL (n = 58) . | HIV-1 Seropositive <200 CD4+ cells/μL (n = 45) . |
|---|---|---|---|
| Absolute Cell Counts (25%, 75%) . | |||
| CD8α+ T cells | 544 (371, 624)* | 1028 (838, 1389) | 762 (422, 932)* |
| CD8αβ+CD8α+ T cells | 463 (345, 560)* | 981 (784, 1300) | 733 (415, 903)* |
| CD8αbright T cells | 461 (331, 552)* | 942 (768, 1289) | 712 (408, 899)* |
| CD8αβ+CD8αbright T cells | 444 (313, 528) | 934 (752, 1276) | 700 (404, 891) |
| NK cells | 260 (155, 429) | 72 (36, 138)* | 82 (53, 163)* |
| CD8α+ NK cells | 154 (72, 190) | 18 (10, 33)* | 30 (11, 41)* |
| CD8αβ+ NK cells | 4 (3, 8) | 1 (0, 3)* | 1 (0, 2)* |
| TCRγδ+ T cells | 74 (37, 128)† | 90 (53, 141)† | 64 (33, 94)† |
| TCRγδ+CD8α+ T cells | 20 (12, 31)* | 40 (22, 58) | 25 (15, 39)* |
| TCRγδ+CD8αβ+ T cells | 3 (1, 5) | 16 (10, 27) | 10 (5, 13) |
| CD4+ T cells | 857 (779, 965) | 408 (330, 525) | 102 (58, 150) |
| CD4+CD8α+ T cells | 12 (8, 14)* | 14 (8, 29)* | 4 (3, 8) |
| CD4+CD8αβ+ T cells | 5 (3, 7)* | 7 (4, 12)* | 3 (2, 5) |
All results are expressed as median absolute cell counts/μL whole blood (25th percentile, 75th percentile). Statistical differences among the three blood donor groups per row were first analyzed by the Kruskal-Wallis test. If the results were found to be significant (P < .05), each of the three data groups per row were analyzed by the Dunn test. Gated cell subsets were defined as: CD8α+ T cells (CD8α+CD3+), CD8αβ+CD8α+ T cells (CD8αβ+CD8α+CD3+), NK cells (CD56+CD3−), TCRγδ+ T cells (TCRγδ+CD3+), and T-helper cells (CD4+CD3+). CD8αbright T cells were defined using a subjective tight gate around the main subpopulation of CD8α+ T cells, which express CD8α in high density as described.32 33
The differences between the two groups were not significant (P < .01) using the Dunn test. All other data groups per row were significantly different.
These data values were not statistically different using the Kruskal-Wallis test.