NGFR Transgene Expression in Transduced Human Cells Recovered From SCID-hu Bone Mice
| Cells Tested . | Engraftment Rate . | % Donor Cells per Graft . | % of Donor Cells That are NGFR+ . |
|---|---|---|---|
| Mock-transduced | 9/10 | 8.5-45.7 | N/A |
| NGFR+CD34+Lin− | 5/5 | 11.6-43.2 | 28.4-44.7 |
| NGFR−CD34+Lin− | 2/2 | 14.4-29.4 | 7.6-12.6 |
| Cells Tested . | Engraftment Rate . | % Donor Cells per Graft . | % of Donor Cells That are NGFR+ . |
|---|---|---|---|
| Mock-transduced | 9/10 | 8.5-45.7 | N/A |
| NGFR+CD34+Lin− | 5/5 | 11.6-43.2 | 28.4-44.7 |
| NGFR−CD34+Lin− | 2/2 | 14.4-29.4 | 7.6-12.6 |
Two days after transduction with the MINGFR retrovirus, CD34+Lin− cells were sorted into NGFR-expressing (NGFR+) and NGFR− subsets and injected into SCID-hu bone grafts. Nine weeks later grafts were analyzed by flow cytometry for donor-derived cells and NGFR transgene expression.
Abbreviation: N/A, not applicable.