Multiplex RT-PCR and Cytogenetic Data on 62 Pediatric ALL Patients
| UPN . | Status . | Diagnose . | Sex . | Age (yr) . | Sample . | Multiplex RT-PCR . | Karyotype . |
|---|---|---|---|---|---|---|---|
| A | |||||||
| 41 | Relapse | T-ALL | M | 22 | PB | t(9;22)(q34;q11) | 46,XY[1]/46-47,XY,del(7)(q11q21),+8,der(11)t(11;14)(p14;q21),14, +21,del(21)(q21)[cp.8] |
| 89 | Presen. | Pre-B-ALL | M | 2.9 | PB | dupMLL(11q23) | 53,XY,+X,+4,+6,+8,+14,+17,+18,+21[co.5] |
| 90□ | Relapse | Pre-B-ALL | M | 9 | PB | t(9;22)(q34;q11) | 46,XY[2]/46,XY,del(12)(p11.1p12.1)[3] |
| 91 | Presen. | Pre-B-ALL | M | 3.3 | BM | t(9;22)(q34;q11) | 46,XY[10] |
| 94 | Relapse | Pre-B-ALL | M | 3.0 | PB | t(9;22)(q34;q11) | 46,XY[4]/46,XY,t(9;22)(q34;q11)[6] |
| 97 | Remis. | Pre-B-ALL | M | 3.4 | PB | dupMLL(11q23) | 53-54,XY,+X,+Y,add(5p),+8,add(8p),+10,+14,+21[co.6] |
| 99 | Presen. | Pre-B-ALL | F | 0.6 | PB | t(10;11)(p12;q23) | 46,XX,add(21)(q?22)[1] |
| 101 | Presen. | Pre-B-ALL | M | 3.9 | PB | dupMLL(11q23) | 46,XY[10] |
| 103 | Relapse | Pre-B-ALL | M | 21 | BM | dupMLL(11q23) | 46-47,XY,del(1)(q25),del(8)(q?21.1q?22),del(10)(q24),+20,+21, [c.10] |
| 108 | Remis. | Pre-B-ALL | M | 9 | BM | dupMLL(11q23) | 46,XY[5] |
| 109 | Presen. | Pre-B-ALL | M | 2.7 | PB | t(12;21)(p13;q22) | Failed |
| 111 | Presen. | Pre-B-ALL | M | 2.4 | PB | dupMLL(11q23) | 46,XY[5] |
| 229 | Presen. | Pre-B-ALL | F | 9 | BM | t(12;21)(p13;q22) | 46,X,del(X)(q?25)[3] |
| 234 | Presen. | Pre-B-ALL | M | 5 | PB | t(4;11)(q21;q23) | 50-51,XY,+Y,+8,+21,+22[cp.3] |
| ? | |||||||
| 243 | Presen. | T-ALL | M | 1.0 | PB | t(11;19)(q23;p13.3) | 46,XY,ins(1)(q21;?)[16]/46,XY,idem,t(14;?)(q11;?)[3]/48,XY, ins(1)(q21;?),+8,+8[3] |
| 253 | Presen. | Pre-B-ALL | M | 3.4 | PB | t(1;19)(q23;p13) | Failed |
| 254 | Presen. | Pre-B-ALL | F | 4.6 | PB | t(12;21)(p13;q22) | Failed |
| 256 | Presen. | Pre-B-ALL | F | 2.6 | PB | t(1;19)(q23;p13) | 46,XY[3]/47,XY,−1,+1p-,+1q-[1] |
| 257 | Remis. | Pre-B-ALL | M | 3.3 | BM | t(1;19)(q23;p13) | 46,XY[10] |
| 262 | Remis. | Pre-B-ALL | F | 7 | BM | t(6;11)(q27;q23) | 46,XX[7]/46,XX,del(6)(q24)[3] |
| 264 | Presen. | Pre-B-ALL | M | 13 | BM | t(6;11)(q27;q23) | Failed |
| 265 | Remis. | Pre-B-ALL | F | 6 | BM | t(11;19)(q23;p13.1) | 46,XX[10] |
| B | |||||||
| 93 | Presen. | Pre-B-ALL | M | 2.5 | PB | t(16;21)(p11;q22) | ND |
| 106 | Presen. | T-ALL | F | 10 | BM | dupMLL(11q23) | ND |
| 112 | Presen. | ALL | M | 10 | BM | dupMLL(11q23) | ND |
| 227 | Presen. | ALL | F | 14 | PB | t(11;19)(q23;p13.1) | ND |
| 238 | Presen. | Pre-B-ALL | F | 10 | BM | t(12;21)(p13;q22) | ND |
| 259 | Remis. | Pre-B-ALL | F | 5 | BM | t(6;11)(q27;q23) | ND |
| C | |||||||
| 95 | Presen. | Pre-B-ALL | M | 8 | PB | NAD | 46,XY[10] |
| 96 | Presen. | Pre-B-ALL | F | 3.9 | PB | NAD | 46,XX[4] |
| 98 | Relapse | ALL | M | 14 | PB | NAD | 46,XY[4] |
| 102 | Presen. | T-ALL | M | 5 | PB | NAD | 46,XY[10] |
| 110 | Presen. | Pre-B-ALL | M | 5 | BM | NAD | 46,XY[1]/46,XY,−9,+14,add(14q)[4] |
| 230 | Presen. | Pre-B-ALL | F | 1.5 | PB | NAD | 46,XX[10] |
| 235 | Presen. | Pre-B-ALL | M | 5 | PB | NAD | 46,XY[3]/44-45,XY,3p-,t(4;6)(p16;q?25),−7,−9[cp.3] |
| 237 | Presen. | T-ALL | F | 6 | BM | NAD | 46,XX[4]/46,XX,del(6)(q26)[4]/46,XX,del(16)(q23)[2] |
| 239 | Presen. | Pre-B-ALL | M | 2.5 | PB | NAD | 46,XY[4] |
| 241 | Presen. | Pre-B-ALL | M | 4.3 | BM | NAD | Failed |
| 242 | Presen. | Pre-B-ALL | M | 7 | PB | NAD | Failed |
| 244 | Presen. | Pre-B-ALL | F | 4.3 | PB | Ins. N/Q cells | 46,XX[10] |
| 245 | Presen. | Pre-B-ALL | M | 4.7 | PB | Ins. N/Q cells | 47,XY,+21[4]/48,XY,+21,+r[2] |
| 248 | Remis. | Pre-B-ALL | F | 5 | BM | Ins. N/Q cells | 46,XX[1] |
| 249 | Presen. | Pro-B-ALL | M | 5 | PB | NAD | 46,XY[9] |
| 250 | Presen. | T-ALL | M | 5 | BM | NAD | Failed |
| 251 | Presen. | Pre-B-ALL | M | 2.0 | PB | NAD | 46,XY[10] |
| 252 | Relapse | Pre-B-ALL | F | 12 | BM | Ins. N/Q cells | Failed |
| 255 | Presen. | Pre-B-ALL | M | 5 | PB | NAD | Failed |
| 258 | Presen. | Pre-B-ALL | F | 5 | PB | NAD | 46,XX[1]/47,XX,+21[3] |
| 260 | Presen. | Pre-B-ALL | M | 3.7 | BM | Ins. N/Q cells | Failed |
| 261 | Presen. | Pre-B-ALL | M | 4.3 | BM | NAD | Failed |
| 263 | Presen. | Pre-B-ALL | F | 6 | BM | NAD | 46,XX[6] |
| D | |||||||
| 88 | Presen. | Pre-B-ALL | F | 4.8 | PB | NAD | ND |
| 92 | Remis. | Pre-B-ALL | M | 7 | BM | NAD | ND |
| 100 | Relapse | Pre-B-ALL | M | 14 | PB | NAD | ND |
| 107 | Presen. | Pre-B-ALL | F | 6 | PB | NAD | ND |
| 228 | Presen. | Pre-B-ALL | M | 3.3 | PB | NAD | ND |
| 231 | Presen. | Pre-B-ALL | F | 7 | PB | NAD | ND |
| 232 | Presen. | Pre-B-ALL | M | 2.2 | BM | NAD | ND |
| 236 | Presen. | Pre-B-ALL | F | 3.2 | PB | NAD | ND |
| 246 | Relapse | Pre-B-ALL | F | 5 | BM | NAD | ND |
| 247 | Presen. | Pre-B-ALL | M | 1.7 | PB | Ins. N/Q cells | ND |
| 320 | Presen. | Pre-B-ALL | F | 2.7 | BM | NAD | ND |
| UPN . | Status . | Diagnose . | Sex . | Age (yr) . | Sample . | Multiplex RT-PCR . | Karyotype . |
|---|---|---|---|---|---|---|---|
| A | |||||||
| 41 | Relapse | T-ALL | M | 22 | PB | t(9;22)(q34;q11) | 46,XY[1]/46-47,XY,del(7)(q11q21),+8,der(11)t(11;14)(p14;q21),14, +21,del(21)(q21)[cp.8] |
| 89 | Presen. | Pre-B-ALL | M | 2.9 | PB | dupMLL(11q23) | 53,XY,+X,+4,+6,+8,+14,+17,+18,+21[co.5] |
| 90□ | Relapse | Pre-B-ALL | M | 9 | PB | t(9;22)(q34;q11) | 46,XY[2]/46,XY,del(12)(p11.1p12.1)[3] |
| 91 | Presen. | Pre-B-ALL | M | 3.3 | BM | t(9;22)(q34;q11) | 46,XY[10] |
| 94 | Relapse | Pre-B-ALL | M | 3.0 | PB | t(9;22)(q34;q11) | 46,XY[4]/46,XY,t(9;22)(q34;q11)[6] |
| 97 | Remis. | Pre-B-ALL | M | 3.4 | PB | dupMLL(11q23) | 53-54,XY,+X,+Y,add(5p),+8,add(8p),+10,+14,+21[co.6] |
| 99 | Presen. | Pre-B-ALL | F | 0.6 | PB | t(10;11)(p12;q23) | 46,XX,add(21)(q?22)[1] |
| 101 | Presen. | Pre-B-ALL | M | 3.9 | PB | dupMLL(11q23) | 46,XY[10] |
| 103 | Relapse | Pre-B-ALL | M | 21 | BM | dupMLL(11q23) | 46-47,XY,del(1)(q25),del(8)(q?21.1q?22),del(10)(q24),+20,+21, [c.10] |
| 108 | Remis. | Pre-B-ALL | M | 9 | BM | dupMLL(11q23) | 46,XY[5] |
| 109 | Presen. | Pre-B-ALL | M | 2.7 | PB | t(12;21)(p13;q22) | Failed |
| 111 | Presen. | Pre-B-ALL | M | 2.4 | PB | dupMLL(11q23) | 46,XY[5] |
| 229 | Presen. | Pre-B-ALL | F | 9 | BM | t(12;21)(p13;q22) | 46,X,del(X)(q?25)[3] |
| 234 | Presen. | Pre-B-ALL | M | 5 | PB | t(4;11)(q21;q23) | 50-51,XY,+Y,+8,+21,+22[cp.3] |
| ? | |||||||
| 243 | Presen. | T-ALL | M | 1.0 | PB | t(11;19)(q23;p13.3) | 46,XY,ins(1)(q21;?)[16]/46,XY,idem,t(14;?)(q11;?)[3]/48,XY, ins(1)(q21;?),+8,+8[3] |
| 253 | Presen. | Pre-B-ALL | M | 3.4 | PB | t(1;19)(q23;p13) | Failed |
| 254 | Presen. | Pre-B-ALL | F | 4.6 | PB | t(12;21)(p13;q22) | Failed |
| 256 | Presen. | Pre-B-ALL | F | 2.6 | PB | t(1;19)(q23;p13) | 46,XY[3]/47,XY,−1,+1p-,+1q-[1] |
| 257 | Remis. | Pre-B-ALL | M | 3.3 | BM | t(1;19)(q23;p13) | 46,XY[10] |
| 262 | Remis. | Pre-B-ALL | F | 7 | BM | t(6;11)(q27;q23) | 46,XX[7]/46,XX,del(6)(q24)[3] |
| 264 | Presen. | Pre-B-ALL | M | 13 | BM | t(6;11)(q27;q23) | Failed |
| 265 | Remis. | Pre-B-ALL | F | 6 | BM | t(11;19)(q23;p13.1) | 46,XX[10] |
| B | |||||||
| 93 | Presen. | Pre-B-ALL | M | 2.5 | PB | t(16;21)(p11;q22) | ND |
| 106 | Presen. | T-ALL | F | 10 | BM | dupMLL(11q23) | ND |
| 112 | Presen. | ALL | M | 10 | BM | dupMLL(11q23) | ND |
| 227 | Presen. | ALL | F | 14 | PB | t(11;19)(q23;p13.1) | ND |
| 238 | Presen. | Pre-B-ALL | F | 10 | BM | t(12;21)(p13;q22) | ND |
| 259 | Remis. | Pre-B-ALL | F | 5 | BM | t(6;11)(q27;q23) | ND |
| C | |||||||
| 95 | Presen. | Pre-B-ALL | M | 8 | PB | NAD | 46,XY[10] |
| 96 | Presen. | Pre-B-ALL | F | 3.9 | PB | NAD | 46,XX[4] |
| 98 | Relapse | ALL | M | 14 | PB | NAD | 46,XY[4] |
| 102 | Presen. | T-ALL | M | 5 | PB | NAD | 46,XY[10] |
| 110 | Presen. | Pre-B-ALL | M | 5 | BM | NAD | 46,XY[1]/46,XY,−9,+14,add(14q)[4] |
| 230 | Presen. | Pre-B-ALL | F | 1.5 | PB | NAD | 46,XX[10] |
| 235 | Presen. | Pre-B-ALL | M | 5 | PB | NAD | 46,XY[3]/44-45,XY,3p-,t(4;6)(p16;q?25),−7,−9[cp.3] |
| 237 | Presen. | T-ALL | F | 6 | BM | NAD | 46,XX[4]/46,XX,del(6)(q26)[4]/46,XX,del(16)(q23)[2] |
| 239 | Presen. | Pre-B-ALL | M | 2.5 | PB | NAD | 46,XY[4] |
| 241 | Presen. | Pre-B-ALL | M | 4.3 | BM | NAD | Failed |
| 242 | Presen. | Pre-B-ALL | M | 7 | PB | NAD | Failed |
| 244 | Presen. | Pre-B-ALL | F | 4.3 | PB | Ins. N/Q cells | 46,XX[10] |
| 245 | Presen. | Pre-B-ALL | M | 4.7 | PB | Ins. N/Q cells | 47,XY,+21[4]/48,XY,+21,+r[2] |
| 248 | Remis. | Pre-B-ALL | F | 5 | BM | Ins. N/Q cells | 46,XX[1] |
| 249 | Presen. | Pro-B-ALL | M | 5 | PB | NAD | 46,XY[9] |
| 250 | Presen. | T-ALL | M | 5 | BM | NAD | Failed |
| 251 | Presen. | Pre-B-ALL | M | 2.0 | PB | NAD | 46,XY[10] |
| 252 | Relapse | Pre-B-ALL | F | 12 | BM | Ins. N/Q cells | Failed |
| 255 | Presen. | Pre-B-ALL | M | 5 | PB | NAD | Failed |
| 258 | Presen. | Pre-B-ALL | F | 5 | PB | NAD | 46,XX[1]/47,XX,+21[3] |
| 260 | Presen. | Pre-B-ALL | M | 3.7 | BM | Ins. N/Q cells | Failed |
| 261 | Presen. | Pre-B-ALL | M | 4.3 | BM | NAD | Failed |
| 263 | Presen. | Pre-B-ALL | F | 6 | BM | NAD | 46,XX[6] |
| D | |||||||
| 88 | Presen. | Pre-B-ALL | F | 4.8 | PB | NAD | ND |
| 92 | Remis. | Pre-B-ALL | M | 7 | BM | NAD | ND |
| 100 | Relapse | Pre-B-ALL | M | 14 | PB | NAD | ND |
| 107 | Presen. | Pre-B-ALL | F | 6 | PB | NAD | ND |
| 228 | Presen. | Pre-B-ALL | M | 3.3 | PB | NAD | ND |
| 231 | Presen. | Pre-B-ALL | F | 7 | PB | NAD | ND |
| 232 | Presen. | Pre-B-ALL | M | 2.2 | BM | NAD | ND |
| 236 | Presen. | Pre-B-ALL | F | 3.2 | PB | NAD | ND |
| 246 | Relapse | Pre-B-ALL | F | 5 | BM | NAD | ND |
| 247 | Presen. | Pre-B-ALL | M | 1.7 | PB | Ins. N/Q cells | ND |
| 320 | Presen. | Pre-B-ALL | F | 2.7 | BM | NAD | ND |
(A) PCR-positive patients with cytogenetic data; (B) PCR-positive patients with no cytogenetic data; (C) PCR-negative patients with cytogenetic data; (D) PCR-negative patients with no cytogenetic data. Cytogenetically t(9;22)(q34;q11)[10] at diagnosis. Abbreviations and notes are as in Table 4.