Table 4.

MCF-7 Clonogenic Cells After Exposure of a Mixture of 9 × 107 MCF-7 Cells in a 100-Fold Excess of HL60 Cells to the CD Adenoviral Vector (200 MOI) for 90 Minutes

MCF-7 Cells Plated at Start of ExperimentColonies Formed at 10-14 Days of 5-FC Incubation
5-FC/CDAV 5-FC Alone CDAV Alone No 5-FC, No CDAV
90 million  None  TMTC  TMTC  TMTC  
10 million None  TMTC  TMTC  TMTC  
2 million  None  1,694 2,800  2,100  
200,000  None  484  520  600 
20,000  None  138  111  146  
2,000  None  34 23  33  
200  None  12  0  4  
20  None  2 0  4 
MCF-7 Cells Plated at Start of ExperimentColonies Formed at 10-14 Days of 5-FC Incubation
5-FC/CDAV 5-FC Alone CDAV Alone No 5-FC, No CDAV
90 million  None  TMTC  TMTC  TMTC  
10 million None  TMTC  TMTC  TMTC  
2 million  None  1,694 2,800  2,100  
200,000  None  484  520  600 
20,000  None  138  111  146  
2,000  None  34 23  33  
200  None  12  0  4  
20  None  2 0  4 

Serial 10-fold dilutions of the cell were followed by plating overnight and rinsing off of the non-adherent cells. The adherent cells were then incubated in 500 μmol/L 5-FC for 10 to 14 days. The colonies were then counted. One colony equals 20 cells.

Abbreviations: TMTC, too many to count; CDAV, cytosine deaminase adenoviral vector.

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