Correlation Between Integrin Expression in Different Cell Types, Infectivity by CD Adenoviral Vector as Measured by Adenoviral Vector β-Galactosidase Activity at 72 Hours After Infection, and Functional Expression of the Adenoviral Vector Cytosine Deaminase Gene as Measured by Conversion of 5-FC to 5-FU During a 24-Hour Period at 72 Hours After Infection With CD Adenoviral Vector
| Cell Type . | αVβ3 . | αVβ5 . | β-Galactosidase Activity at 72 h (% of geo. mean) . | 5-FC Conversion (%) . |
|---|---|---|---|---|
| CD34+ | 4.4% | 3.6% | — | — |
| MDA-MB-453 | 0.4% | 15.8% | 38.6 | 15.9 |
| T47D | 0.8% | 96.4% | 586 | 51 |
| MCF-7 | 0.6% | 90.2% | 466.9 | 38.2 |
| MDA-MB-231 | 42.5% | 89.4% | — | — |
| MDA-MB-468 | 3.3% | 90.3% | — | — |
| BT-20 | 0% | 92.5% | — | — |
| MDA-MB-436 | 0% | 33.8% | — | — |
| Cell Type . | αVβ3 . | αVβ5 . | β-Galactosidase Activity at 72 h (% of geo. mean) . | 5-FC Conversion (%) . |
|---|---|---|---|---|
| CD34+ | 4.4% | 3.6% | — | — |
| MDA-MB-453 | 0.4% | 15.8% | 38.6 | 15.9 |
| T47D | 0.8% | 96.4% | 586 | 51 |
| MCF-7 | 0.6% | 90.2% | 466.9 | 38.2 |
| MDA-MB-231 | 42.5% | 89.4% | — | — |
| MDA-MB-468 | 3.3% | 90.3% | — | — |
| BT-20 | 0% | 92.5% | — | — |
| MDA-MB-436 | 0% | 33.8% | — | — |
5-FC conversion: 72 hours after exposure to the CD adenoviral vector, 6-(3H) 5-FC is added to the culture for 24 hours. The cellular extract is then subjected to HPLC to measure conversion by the cells from 5-FC to 5-FU. β-Galactosidase enzymatic assay was assayed 72 hours after exposure to the CD adenoviral vector (72 hours was allowed to elapse to permit time for viral transgene expression).