Influence of GM-CSF on EBV-Induced Apoptosis in Neutrophils
| . | Treatments . | |||
|---|---|---|---|---|
| Control . | GM-CSF . | EBV . | EBV + GM-CSF . | |
| Donor 1 | 26 | 15 | 65 | 25 |
| Donor 2 | 28 | 17 | 57 | 31 |
| Donor 3 | 23 | 14 | 43 | 9 |
| Donor 4 | 38 | 21 | 68 | 42 |
| Mean of apoptotic cells ± SD | 29 ± 7 | 17 ± 3-150 | 58 ± 11 | 27 ± 14-150 |
| . | Treatments . | |||
|---|---|---|---|---|
| Control . | GM-CSF . | EBV . | EBV + GM-CSF . | |
| Donor 1 | 26 | 15 | 65 | 25 |
| Donor 2 | 28 | 17 | 57 | 31 |
| Donor 3 | 23 | 14 | 43 | 9 |
| Donor 4 | 38 | 21 | 68 | 42 |
| Mean of apoptotic cells ± SD | 29 ± 7 | 17 ± 3-150 | 58 ± 11 | 27 ± 14-150 |
PMN (2 × 106/mL) were incubated with EBV, GM-CSF (3 nmol/L), or with both agonists for 20 hours. Cells were then stained and the number of apoptotic cells was evaluated by flow cytometry, as described in Materials and Methods. Results are expressed in percentage (%) of apoptotic cells for each culture performed in duplicate. The mean of apoptotic cells from GM-CSF–treated cells was compared with that obtained from control cells, and the mean of apoptotic cells from EBV + GM-CSF–treated cells was compared with that obtained from EBV-treated cells.
Significantly different at P < .01.