Differential Blood Leukocyte Analysis of BCG-Infected IFN-γ −/− Mice
| . | Genotype . | Strain* . | No. . | WBC/μL-151 . | Lymphocytes . | Polys-152 . | Monocytes . | Eosinophils . |
|---|---|---|---|---|---|---|---|---|
| Naive mice | +/+ | C57BL/6 | 8 | 9,000 | 74.5 (4.8) | 23.9 (6.7) | 2 (1.4) | 1.7 (1.3) |
| −/− | C57BL/6 | 8 | 3,562 | 76.3 (0.6) | 20.6 (0.8) | 1.5 (2) | 0 (0) | |
| +/+ | Balb/c | 8 | 3,942 | 79.2 (6.0) | 14.2 (5.0) | 5.4 (4.3) | 0.7 (1.1) | |
| −/− | Balb/c | 9 | 3,213 | 82.2 (6.2) | 14.6 (6.6) | 2.8 (1.7) | 0.8 (1.9) | |
| BCG-infected mice | ||||||||
| Experiment | ||||||||
| 1 | +/+ | C57BL/6 | 5 | 6,500 | 81.7 (4.9) | 13.0 (2.0) | 5.3 (3.2) | 0 (0) |
| −/− | C57BL/6 | 5 | 18,750 | 10.3 (4.0) | 78.3 (2.5) | 10.7 (1.2) | 0.7 (0.5) | |
| 2 | +/+ | C57BL/6 | 5 | 4,750 | 73.4 (6.5) | 16.4 (4.9) | 9.6 (3.0) | 0.5 (0.9) |
| −/− | C57BL/6 | 5 | 58,250 | 7.0 (3.5) | 84.7 (4.9) | 8.3 (1.5) | 0 (0) | |
| 3 | +/+ | Balb/c | 5 | 3,433 | 77.7 (10.4) | 9.2 (2.1) | 6.8 (3.8) | 0.4 (0.7) |
| −/− | Balb/c | 5 | 80,166 | 4.8 (5.4) | 85.8 (9.9) | 9.0 (4.6) | 0 (0) |
| . | Genotype . | Strain* . | No. . | WBC/μL-151 . | Lymphocytes . | Polys-152 . | Monocytes . | Eosinophils . |
|---|---|---|---|---|---|---|---|---|
| Naive mice | +/+ | C57BL/6 | 8 | 9,000 | 74.5 (4.8) | 23.9 (6.7) | 2 (1.4) | 1.7 (1.3) |
| −/− | C57BL/6 | 8 | 3,562 | 76.3 (0.6) | 20.6 (0.8) | 1.5 (2) | 0 (0) | |
| +/+ | Balb/c | 8 | 3,942 | 79.2 (6.0) | 14.2 (5.0) | 5.4 (4.3) | 0.7 (1.1) | |
| −/− | Balb/c | 9 | 3,213 | 82.2 (6.2) | 14.6 (6.6) | 2.8 (1.7) | 0.8 (1.9) | |
| BCG-infected mice | ||||||||
| Experiment | ||||||||
| 1 | +/+ | C57BL/6 | 5 | 6,500 | 81.7 (4.9) | 13.0 (2.0) | 5.3 (3.2) | 0 (0) |
| −/− | C57BL/6 | 5 | 18,750 | 10.3 (4.0) | 78.3 (2.5) | 10.7 (1.2) | 0.7 (0.5) | |
| 2 | +/+ | C57BL/6 | 5 | 4,750 | 73.4 (6.5) | 16.4 (4.9) | 9.6 (3.0) | 0.5 (0.9) |
| −/− | C57BL/6 | 5 | 58,250 | 7.0 (3.5) | 84.7 (4.9) | 8.3 (1.5) | 0 (0) | |
| 3 | +/+ | Balb/c | 5 | 3,433 | 77.7 (10.4) | 9.2 (2.1) | 6.8 (3.8) | 0.4 (0.7) |
| −/− | Balb/c | 5 | 80,166 | 4.8 (5.4) | 85.8 (9.9) | 9.0 (4.6) | 0 (0) |
*Background strain onto which the IFN-γ−/− mutation has been backcrossed.
Average leukocyte counts. All other values are average with the standard deviation in brackets.
Polymorphonuclear cells including neutrophils and band forms counted together. Note that −/− mice have 10% to 40% band forms in the peripheral blood.