Infection of Human Primary T Lymphocytes With GaLV– and VSV-G–Enveloped Virions
| Viral Dilution: . | GaLV: . | VSV-G: . | ||
|---|---|---|---|---|
| . | m.o.i.* . | % NTP+ . | m.o.i.† . | % NTP+ . |
| 1 | 0.9 | 24 ± 5% | 150 | 18 ± 3% |
| 1:10 | 9 × 10−2 | 6 ± 3% | 15 | 11 ± 2% |
| 1:100 | 9 × 10−3 | 1 ± 1% | 1.5 | 6 ± 2% |
| 1:1,000 | 9 × 10−4 | 0 ± 1% | 0.15 | 1 ± 1% |
| Viral Dilution: . | GaLV: . | VSV-G: . | ||
|---|---|---|---|---|
| . | m.o.i.* . | % NTP+ . | m.o.i.† . | % NTP+ . |
| 1 | 0.9 | 24 ± 5% | 150 | 18 ± 3% |
| 1:10 | 9 × 10−2 | 6 ± 3% | 15 | 11 ± 2% |
| 1:100 | 9 × 10−3 | 1 ± 1% | 1.5 | 6 ± 2% |
| 1:1,000 | 9 × 10−4 | 0 ± 1% | 0.15 | 1 ± 1% |
One million mitogen-activated T lymphocytes were infected overnight with 10-fold serial dilutions of cell-free viral stocks in the presence of polybrene (4 μg/mL) as described in the text. The cultures were stained for CD3 and NTP 10 days after infection as described in Fig 2. The mean ± SD of the percent NTP+ cells among all CD3+ cells is calculated from three independent experiments.
At the first dilution, the virion concentration is 9 × 105 infectious particles/mL (see A549, Fig 1).
The first dilution is a 1:200 dilution of a stock containing 3 × 1010 infectious particles/mL (see A549, Fig 1).