Table 1. MRA of HSC Subsets Isolated by... | American Society of Hematology

Table 1.

MRA of HSC Subsets Isolated by Fluorescent Staining With Rh-123, JC-1, or NAO

Cells Transplanted	Cells/Femur	Low/High Ratio	CFU-S/Femur	Low/High Ratio
Experiment No. 1
None	3.1 × 10⁵		4.7 ± 1.1
Rh-123^low	5.3 × 10⁶		115 ± 78^-150
Rh-123^high	2.2 × 10⁶	2.41	4.4 ± 2.4	26.1
JC-1^low	1.6 × 10⁷		206 ± 66
JC-1^high	2.8 × 10⁶	5.71	0.6 ± 1.3	343
Experiment No. 2
None	4.7 × 10⁵		3.4 ± 0.3
Rh-123^low	1.3 × 10⁷		425 ± 202
Rh-123^high	3.3 × 10⁶	3.94	3.8 ± 2.5	112
NAO^low	4.6 × 10⁶		49 ± 8.5
NAO^high	4.8 × 10⁶	0.96	75 ± 18^-151	0.65

Cells Transplanted	Cells/Femur	Low/High Ratio	CFU-S/Femur	Low/High Ratio
Experiment No. 1
None	3.1 × 10⁵		4.7 ± 1.1
Rh-123^low	5.3 × 10⁶		115 ± 78^-150
Rh-123^high	2.2 × 10⁶	2.41	4.4 ± 2.4	26.1
JC-1^low	1.6 × 10⁷		206 ± 66
JC-1^high	2.8 × 10⁶	5.71	0.6 ± 1.3	343
Experiment No. 2
None	4.7 × 10⁵		3.4 ± 0.3
Rh-123^low	1.3 × 10⁷		425 ± 202
Rh-123^high	3.3 × 10⁶	3.94	3.8 ± 2.5	112
NAO^low	4.6 × 10⁶		49 ± 8.5
NAO^high	4.8 × 10⁶	0.96	75 ± 18^-151	0.65

Mouse HSC isolated by the Thy-1.1^lowSca-1⁺Lin⁻ phenotype were further separated based on levels of JC-1, NAO, or Rh-123 staining. Cells in the upper 30% and lower 30% of staining intensity were isolated in each case, and approximately 2,000 cells of each population were transplanted into two lethally irradiated syngeneic mice. Twelve to 13 days later the cellularity of the femurs was determined; the numbers indicate the cell count derived from four pooled femurs. CFU-S/femur indicates the content of day 13 CFU-S per femur in the primary transplant recipients (mean ± SD), as measured in groups of four secondary recipient mice except as noted.

F0-150

Eight secondary recipients analyzed.

F0-151

Three secondary recipients analyzed.

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