Transactivation Properties of Different Retinoids
| . | TRE2-tk-LUC . | (βRARE)3-tk-LUC . | ||||||
|---|---|---|---|---|---|---|---|---|
| . | EC50 (nmol/L) . | EC50 (nmol/L) . | ||||||
| . | t-RA . | 9cisRA . | AM580 . | TTNPB . | t-RA . | 9cisRA . | AM580 . | TTNPB . |
| RARα | 1.6 | 367.9 | 0.2 | 0.03 | 0.4 | 6.6 | 0.4 | 0.002 |
| bcr1-PML/RARα | 0.5 | 302.6 | 0.2 | 0.02 | 0.8 | 28.9 | 0.5 | 0.002 |
| bcr3-PML/RARα | 0.3 | 37.5 | 0.4 | 0.15 | 0.9 | 28.9 | 2.7 | 0.015 |
| . | TRE2-tk-LUC . | (βRARE)3-tk-LUC . | ||||||
|---|---|---|---|---|---|---|---|---|
| . | EC50 (nmol/L) . | EC50 (nmol/L) . | ||||||
| . | t-RA . | 9cisRA . | AM580 . | TTNPB . | t-RA . | 9cisRA . | AM580 . | TTNPB . |
| RARα | 1.6 | 367.9 | 0.2 | 0.03 | 0.4 | 6.6 | 0.4 | 0.002 |
| bcr1-PML/RARα | 0.5 | 302.6 | 0.2 | 0.02 | 0.8 | 28.9 | 0.5 | 0.002 |
| bcr3-PML/RARα | 0.3 | 37.5 | 0.4 | 0.15 | 0.9 | 28.9 | 2.7 | 0.015 |
Extracts were prepared from transiently cotransfected COS-1 cells and analyzed as described in Materials and Methods. The EC50 values (retinoid concentrations required to induce 50% of the maximal response) were calculated using the nonlinear test squares regression analysis program ALLFIT.