Retinoid Binding Specificity of RARα, bcr1-, and bcr3-PML/RARα
| Retinoid . | EC50 (nmol/L) . | ||
|---|---|---|---|
| . | RARα . | PML/RARα . | PML/RARα . |
| . | . | bcr1 . | bcr3 . |
| t-RA | 4.9 ± 0.7 | 4.8 ± 0.3 | 7.5 ± 0.2 |
| 9-cis-RA | 29.5 ± 5.5 | 27.5 ± 4.1 | 4.8 ± 1.3 |
| Ch55 | 15.9 ± 7.1 | 19.7 ± 4.5 | 18.3 ± 5.2 |
| AM580 | 18.1 ± 1.3 | 39.1 ± 3.2 | 22.2 ± 5.4 |
| TTNPB | 52.6 ± 8.3 | 21.5 ± 9.2 | 33.1 ± 7.4 |
| Retinoid . | EC50 (nmol/L) . | ||
|---|---|---|---|
| . | RARα . | PML/RARα . | PML/RARα . |
| . | . | bcr1 . | bcr3 . |
| t-RA | 4.9 ± 0.7 | 4.8 ± 0.3 | 7.5 ± 0.2 |
| 9-cis-RA | 29.5 ± 5.5 | 27.5 ± 4.1 | 4.8 ± 1.3 |
| Ch55 | 15.9 ± 7.1 | 19.7 ± 4.5 | 18.3 ± 5.2 |
| AM580 | 18.1 ± 1.3 | 39.1 ± 3.2 | 22.2 ± 5.4 |
| TTNPB | 52.6 ± 8.3 | 21.5 ± 9.2 | 33.1 ± 7.4 |
Nuclear extracts prepared from RARα, bcr1-, and bcr3-PML/RARα transiently transfected COS-1 cells were labeled for 18 hours at 4°C with 10 nmol/L [3H]-tRA in the presence of increasing concentrations (0.5 nmol/L to 1 μmol/L) of the indicated unlabeled retinoid. RA binding was analysed using PD10 desalting columns (Pharmacia). The EC50 values were calculated using the nonlinear least squares regression analysis program ALLFIT. Values are means for at least three determinations ± SD.