Determination of FasL Expression (MFI) in Lymphocytic Subsets of B-CLL Patients and Healthy Individuals
Lymphocytic Subset
.
B-CLL Patients
.
P
.
Healthy Donors
.
CD4+
4.2 ± .63
<.009
2.1 ± .5
CD8+
2.8 ± .29
>.2
2.0 ± .7
CD19+
2.4 ± .21
>.9
2.3 ± .1
Lymphocytic Subset
.
B-CLL Patients
.
P
.
Healthy Donors
.
CD4+
4.2 ± .63
<.009
2.1 ± .5
CD8+
2.8 ± .29
>.2
2.0 ± .7
CD19+
2.4 ± .21
>.9
2.3 ± .1
MFI was determined by immunofluorescence staining and FACS analysis and calculated as the ratio of the MFI achieved with anti FasL MoAb/isotype control Moab. MFI >1.5 was considered positive. Means ± SEM of 30 B-CLL patients and 20 healthy individuals are given.